Overview

  • Product name

  • Description

    Rabbit polyclonal to RAB31
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Human RAB31 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: Q13636

  • Positive control

    • IHC-P: Human colon MALT and vessels tissue. ICC/IF: RAW 264.7 cells. WB: MCF7 and RAW 264.7 whole cell lysates; Rat brain lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 30% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 22 kDa.
ICC/IF 1/100 - 1/500.
IHC-P 1/100.

Target

Images

  • All lanes : Anti-RAB31 antibody (ab230881) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : Rat brain lysate

    Predicted band size: 22 kDa

  • RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for RAB31 using ab230881 at 1/100 dilution in ICC/IF.

    Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • Formalin-fixed, paraffin-embedded human colon, MALT and vessels stained for RAB31 using ab230881 at 1/100 dilution in immunohistochemical analysis.

References

ab230881 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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