Anti-RAB31 antibody (ab230881)
Key features and details
- Rabbit polyclonal to RAB31
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-RAB31 antibody
See all RAB31 primary antibodies -
Description
Rabbit polyclonal to RAB31 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig -
Immunogen
Synthetic peptide corresponding to Human RAB31 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q13636 -
Positive control
- IHC-P: Human colon MALT and vessels tissue. ICC/IF: RAW 264.7 cells. WB: MCF7 and RAW 264.7 whole cell lysates; Rat brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 22 kDa.
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ICC/IF |
1/100 - 1/500.
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IHC-P |
1/100.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 22 kDa. |
ICC/IF
1/100 - 1/500. |
IHC-P
1/100. |
Target
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Tissue specificity
Highest expression in placenta and brain with lower levels in heart and lung. Not detected in liver, skeletal muscle, kidney or pancreas. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 616979 Cow
- Entrez Gene: 11031 Human
- Entrez Gene: 106572 Mouse
- Entrez Gene: 100737127 Pig
- Entrez Gene: 246324 Rat
- Omim: 605694 Human
- SwissProt: Q13636 Human
- SwissProt: Q921E2 Mouse
see all -
Alternative names
- Rab 22B antibody
- RAB 31 antibody
- Rab22B antibody
see all
Images
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All lanes : Anti-RAB31 antibody (ab230881) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : Rat brain lysate
Predicted band size: 22 kDa -
RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for RAB31 using ab230881 at 1/100 dilution in ICC/IF.
Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Formalin-fixed, paraffin-embedded human colon, MALT and vessels stained for RAB31 using ab230881 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab230881 has been referenced in 2 publications.
- Li X et al. High expression of Rab31 confers a poor prognosis and enhances cell proliferation and invasion in oral squamous cell carcinoma. Oncol Rep 45:1182-1192 (2021). PubMed: 33469675
- Wu Q et al. RAB31 is targeted by miR-26b and serves a role in the promotion of osteosarcoma. Oncol Lett 20:244 (2020). PubMed: 32973957