Overview

  • Product name

  • Description

    Rabbit polyclonal to RAB32
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length Human RAB32 (NP_006825.1; amino acids 1-225).

  • Positive control

    • Human liver lysate; RAB32 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab103160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa.

Target

  • Function

    Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission.
  • Tissue specificity

    Widely expressed with high levels in heart, liver, kidney, bone marrow, testis, colon and fetal lung.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • RAB32 antibody
    • RAB32, member RAS oncogene family antibody
    • RAB32_HUMAN antibody
    • Ras related protein Rab32 antibody
    • Ras-related protein Rab-32 antibody
    see all

Images

  • Anti-RAB32 antibody (ab103160) at 1 µg/ml + Human liver lysate at 50 µg

    Predicted band size: 25 kDa

  • All lanes : Anti-RAB32 antibody (ab103160) at 1 µg/ml

    Lane 1 : RAB32 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 25 kDa

References

ab103160 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T, HCT116)
Gel Running Conditions
Reduced Denaturing
Loading amount
35 µg
Specification
HEK293T, HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 31 2019

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