Anti-RAB32 antibody (ab229604)
Key features and details
- Rabbit polyclonal to RAB32
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-RAB32 antibody
See all RAB32 primary antibodies -
Description
Rabbit polyclonal to RAB32 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey -
Immunogen
Recombinant fragment within Human RAB32 (internal sequence). The exact sequence is proprietary.
Database link: Q13637 -
Positive control
- IP: HeLa whole cell extracts. WB: A431, HeLa and HepG2 whole cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 25 kDa.
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IP |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 25 kDa. |
IP
1/100 - 1/500. |
Target
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Function
Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission. -
Tissue specificity
Widely expressed with high levels in heart, liver, kidney, bone marrow, testis, colon and fetal lung. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 10981 Human
- Entrez Gene: 702431 Rhesus monkey
- Omim: 612906 Human
- SwissProt: Q13637 Human
- Unigene: 287714 Human
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Alternative names
- RAB32 antibody
- RAB32, member RAS oncogene family antibody
- RAB32_HUMAN antibody
see all
Images
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All lanes : Anti-RAB32 antibody (ab229604) at 1/1000 dilution
Lane 1 : A431 (Human epidermoid carcinoma cell line) whole cell extract
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa12% SDS-PAGE gel.
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RAB32 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 5 µg ab229604. Western blot was performed from the immunoprecipitate using ab229604. Anti-Rabbit IgG was used as a secondary antibody.
Lane 1: HeLa whole cell lysate (Input).
Lane 2: Control IgG IP in HeLa whole cell lysate.
Lane 3: ab229604 IP in HeLa whole cell lysate.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229604 has not yet been referenced specifically in any publications.