Key features and details
- Rabbit polyclonal to RAB32
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-RAB32 antibody
See all RAB32 primary antibodies
DescriptionRabbit polyclonal to RAB32
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human RAB32 (internal sequence). The exact sequence is proprietary.
Database link: Q13637
- IP: HeLa whole cell extracts. WB: A431, HeLa and HepG2 whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 25 kDa.|
|IP||1/100 - 1/500.|
FunctionActs as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission.
Tissue specificityWidely expressed with high levels in heart, liver, kidney, bone marrow, testis, colon and fetal lung.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
- Information by UniProt
- RAB32 antibody
- RAB32, member RAS oncogene family antibody
- RAB32_HUMAN antibody
All lanes : Anti-RAB32 antibody (ab229604) at 1/1000 dilution
Lane 1 : A431 (Human epidermoid carcinoma cell line) whole cell extract
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 25 kDa
12% SDS-PAGE gel.
RAB32 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 5 µg ab229604. Western blot was performed from the immunoprecipitate using ab229604. Anti-Rabbit IgG was used as a secondary antibody.
Lane 1: HeLa whole cell lysate (Input).
Lane 2: Control IgG IP in HeLa whole cell lysate.
Lane 3: ab229604 IP in HeLa whole cell lysate.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229604 has not yet been referenced specifically in any publications.