Key features and details
- Rabbit polyclonal to Rab34
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Rab34 antibody
See all Rab34 primary antibodies
DescriptionRabbit polyclonal to Rab34
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
Synthetic peptide corresponding to Human Rab34 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human Rab34 protein (ab115717) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Tissue: Mouse brain; Human liver; Rat brain. Whole cell: A431; F9 It also gave a positive signal in FFPE human normal lung tissue sections.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab73383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionProtein transport. Involved in the redistribution of lysosomes to the peri-Golgi region.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationCytoplasm. Golgi apparatus. Associates with the Golgi complex.
- Information by UniProt
- Rab-39 antibody
- RAB34 antibody
- RAB34, member RAS oncogene family antibody
All lanes : Anti-Rab34 antibody (ab73383) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Brain (Rat) Tissue Lysate
Lane 5 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Additional bands at: 18 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ICC/IF image of ab73383 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73383, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa cells at 1µg/ml.
IHC image of Rab34 staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73383, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab73383 has been referenced in 2 publications.
- Starling GP et al. Folliculin directs the formation of a Rab34-RILP complex to control the nutrient-dependent dynamic distribution of lysosomes. EMBO Rep 17:823-41 (2016). PubMed: 27113757
- Kasmapour B et al. Size-dependent mechanism of cargo sorting during lysosome-phagosome fusion is controlled by Rab34. Proc Natl Acad Sci U S A 109:20485-90 (2012). WB . PubMed: 23197834