• Product name

  • Description

    Rabbit polyclonal to RAB35
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 71-120 (RTITSTYYRG THGVIVVYDV TSAESFVNVK RWLHEINQNC DDVCRILVGN) of Human RAB35 (NP_006852).



Our Abpromise guarantee covers the use of ab105762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-Fr Use at an assay dependent concentration. PubMed: 23115302


  • Function

    In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Cell membrane. Membrane > clathrin-coated pit. Cytoplasmic vesicle > clathrin-coated vesicle. Endosome. Melanosome. Present on sorting endosomes and endosomal tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • GTP binding protein RAY antibody
    • GTP-binding protein RAY antibody
    • H ray antibody
    • OTTHUMP00000240256 antibody
    • OTTHUMP00000240257 antibody
    • OTTHUMP00000240258 antibody
    • OTTHUMP00000240259 antibody
    • RAB1C antibody
    • Rab35 antibody
    • RAB35 member RAS oncogene family antibody
    • RAB35, member RAS oncogene family antibody
    • RAB35_HUMAN antibody
    • Ras related protein rab 1c (GTP binding protein ray) antibody
    • Ras related protein Rab 1C antibody
    • Ras related protein Rab35 antibody
    • Ras-related protein Rab-1C antibody
    • Ras-related protein Rab-35 antibody
    • RAY antibody
    see all


  • Immunohistochemical analysis of frozen serial sections of skeletal muscle biopsies from Human filaminopathy patients, staining RAB35 with ab105762.

    Sections were incubated overnight at 4°C with primary antibody (1/500) and washed three times with PBS for 5 min. Subsequently, sections were DyLight®488-conjugated anti-rabbit IgG (1/1000).

  • Anti-RAB35 antibody (ab105762) (0.2-1 µg/ml) + Human Liver

    Predicted band size: 23 kDa


This product has been referenced in:

  • Lesteberg K  et al. Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunol Res 65:1031-1045 (2017). Flow Cyt ; Human . Read more (PubMed: 28822075) »
  • Kley RA  et al. A combined laser microdissection and mass spectrometry approach reveals new disease relevant proteins accumulating in aggregates of filaminopathy patients. Mol Cell Proteomics 12:215-27 (2013). IHC-Fr ; Human . Read more (PubMed: 23115302) »
See all 2 Publications for this product

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