Overview

  • Product name

  • Description

    Rabbit polyclonal to RAB35
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human RAB35 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: Q15286

  • Positive control

    • IHC-P: Human liver and brain cortex tissues. WB: MCF7, RAW 264.7 and PC-12 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab230838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 23 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Cell membrane. Membrane > clathrin-coated pit. Cytoplasmic vesicle > clathrin-coated vesicle. Endosome. Melanosome. Present on sorting endosomes and endosomal tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • GTP binding protein RAY antibody
    • GTP-binding protein RAY antibody
    • H ray antibody
    • OTTHUMP00000240256 antibody
    • OTTHUMP00000240257 antibody
    • OTTHUMP00000240258 antibody
    • OTTHUMP00000240259 antibody
    • RAB1C antibody
    • Rab35 antibody
    • RAB35 member RAS oncogene family antibody
    • RAB35, member RAS oncogene family antibody
    • RAB35_HUMAN antibody
    • Ras related protein rab 1c (GTP binding protein ray) antibody
    • Ras related protein Rab 1C antibody
    • Ras related protein Rab35 antibody
    • Ras-related protein Rab-1C antibody
    • Ras-related protein Rab-35 antibody
    • RAY antibody
    see all

Images

  • All lanes : Anti-RAB35 antibody (ab230838) at 1/500 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Predicted band size: 23 kDa

  • Formalin-fixed, paraffin-embedded human liver tissue stained for RAB35 using ab230838 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human brain cortex tissue stained for RAB35 using ab230838 at 1/100 dilution in immunohistochemical analysis.

References

ab230838 has not yet been referenced specifically in any publications.

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