Recombinant Anti-RAB39B antibody [EPR9651] - BSA and Azide free (ab249132)
![Immunocytochemistry - Anti-RAB39B antibody [EPR9651] - BSA and Azide free (ab249132)](https://www.abcam.com/ps/products/249/ab249132/Images/ab249132-1-anti-rab39b-antibody-epr9651-immunocytochemistry-mouse-primary-neuron-cell8.jpg "Immunocytochemistry - Anti-RAB39B antibody [EPR9651] - BSA and Azide free (ab249132)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9651] to RAB39B - BSA and Azide free
- Suitable for: ICC, Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-RAB39B antibody [EPR9651] - BSA and Azide free
See all RAB39B primary antibodies -
Description
Rabbit monoclonal [EPR9651] to RAB39B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, Flow Cyt (Intra), WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: Mouse primary neuron cells.
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General notes
ab249132 is the carrier-free version of ab154826.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9651 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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| Notes |
|---|
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ICC
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
Target
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Relevance
RAB39B is a member of the Rab family of proteins. Rab proteins are small GTPases that are involved in vesicular trafficking. -
Cellular localization
Cell membrane; Lipid-anchor; Cytoplasmic side -
Database links
- Entrez Gene: 116442 Human
- Entrez Gene: 67790 Mouse
- Omim: 300774 Human
- SwissProt: Q96DA2 Human
- SwissProt: Q8BHC1 Mouse
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Alternative names
- 6330580M05Rik antibody
- MRX72 antibody
- OTTHUMP00000024247 antibody
see all
Images
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Immunocytochemistry - Anti-RAB39B antibody [EPR9651] - BSA and Azide free (ab249132)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling RAB39B with ab154826 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in mouse primary neuron cell.Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154826).
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![Anti-RAB39B antibody [EPR9651] - BSA and Azide free (ab249132)](https://www.abcam.com/ps/products/249/ab249132/Images/ab249132-2-benefits-of-recombinant-antibodies.png)
Anti-RAB39B antibody [EPR9651] - BSA and Azide free (ab249132)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249132 has not yet been referenced specifically in any publications.
