Key features and details
- Mouse monoclonal [6D1A12] to Rab3A
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Rab3A antibody [6D1A12]
See all Rab3A primary antibodies
DescriptionMouse monoclonal [6D1A12] to Rab3A
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Cynomolgus monkey
Recombinant full length protein corresponding to Human Rab3A aa 1-220. Expressed in E.coli.
MASATDSRYGQKESSDQNFDYMFKILIIGNSSVGKTSFLFRYADDSFTPA FVSTVGIDFKVKTIYRNDKRIKLQIWDTAGQERYRTITTAYYRGAMGFIL MYDITNEESFNAVQDWSTQIKTYSWDNAQVLLVGNKCDMEDERVVSSERG RQLADHLGFEFFEASAKDNINVKQTFERLVDVICEKMSESLDTADPAVTG AKQGPQLSDQQVPPHQDCAC
Database link: P20336
- WB: Recombinant human Rab3A (aa 1-220) protein; Rab3A (aa 1-220)-hIgGFc-transfected HEK-293 cell lysate. Flow cyt: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab234089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500.|
|Flow Cyt||1/200 - 1/400.|
FunctionInvolved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
Tissue specificitySpecifically expressed in brain.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationCell membrane.
- Information by UniProt
- Rab 3A antibody
- RAB 3A member RAS oncogene family antibody
- Rab3a antibody
Anti-Rab3A antibody [6D1A12] (ab234089) at 1/100 dilution + Recombinant human Rab3A (aa 1-220) protein
Expected recombinant MW is 51 kDa.
All lanes : Anti-Rab3A antibody [6D1A12] (ab234089) at 1/100 dilution
Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : Rab3A (aa 1-220)-hIgGFc-transfected HEK-293 cell lysate cell lysate
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Rab3A with ab234089 at 1/200 dilution (green) compared to a negative control (red).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234089 has not yet been referenced specifically in any publications.