Key features and details
- Rabbit polyclonal to Rab3A
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Rab3A antibody
See all Rab3A primary antibodies
DescriptionRabbit polyclonal to Rab3A
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- ICC: HeLa cells. WB: Mouse and rat brain tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 0.1% BSA
Concentration information loading...
PurityProtein A purified
Primary antibody notesRab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in co-ordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals and the best studied is rab 3A. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa. Abnormal accumulation of rab 3A in the cytoplasm of Purkinje cells has been reported in the prion protein related Creutzfeldt-Jakob disease.
Our Abpromise guarantee covers the use of ab3335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml.|
|ICC/IF||Use a concentration of 2 µg/ml.|
FunctionInvolved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
Tissue specificitySpecifically expressed in brain.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationCell membrane.
- Information by UniProt
- Rab 3A antibody
- RAB 3A member RAS oncogene family antibody
- Rab3a antibody
Immunofluorescence analysis of RAB3A was done on 70% confluent log phase HeLa (Human epithelial adenocarcinoma cell line)
cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3335 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution. Panel d is a merged image showing Cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
All lanes : Anti-Rab3A antibody (ab3335) at 2 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat kidney tissue lysate
Lane 6 : Rat liver tissue lysate
Lysates/proteins at 30 µg per lane.
All lanes : Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
A 27 kDa band corresponding to RAB3A was observed along with two uncharacterized band (*) at ~40 kD and 60 kDa across tissues tested.
ab3335 has been referenced in 11 publications.
- Connor-Robson N et al. An integrated transcriptomics and proteomics analysis reveals functional endocytic dysregulation caused by mutations in LRRK2. Neurobiol Dis 127:512-526 (2019). PubMed: 30954703
- Folsom TD et al. Quantitative proteomics of forebrain subcellular fractions in fragile X mental retardation 1 knockout mice following acute treatment with 2-Methyl-6-(phenylethynyl)pyridine: Relevance to developmental study of schizophrenia. Synapse 73:e22069 (2019). PubMed: 30176067
- Wang Z et al. Effects of exercise-induced fatigue on the morphology of asymmetric synapse and synaptic protein levels in rat striatum. Neurochem Int 129:104476 (2019). PubMed: 31145967
- Spencer B et al. Reducing Endogenous a-Synuclein Mitigates the Degeneration of Selective Neuronal Populations in an Alzheimer's Disease Transgenic Mouse Model. J Neurosci 36:7971-84 (2016). IHC-FrFl ; Mouse . PubMed: 27466341
- Mohankumar KM et al. An in vivo screen identifies ependymoma oncogenes and tumor-suppressor genes. Nat Genet 47:878-87 (2015). WB . PubMed: 26075792