Overview

  • Product name

  • Description

    Rabbit polyclonal to Rab3A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Hamster, Dog, Human
    Predicted to work with: Cow, Pig, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human Rab3A aa 1-18.
    Sequence:

    MASATDSRYGQKESSDQN


    (Peptide available as ab4951)

  • Positive control

    • Hepa1 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Primary antibody notes

    Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in co-ordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals and the best studied is rab 3A. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa. Abnormal accumulation of rab 3A in the cytoplasm of Purkinje cells has been reported in the prion protein related Creutzfeldt-Jakob disease.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr 1/300.

Target

Images

  • Western blot detection of Rab3A on Hepa1 cell lysate using ab3335.

  • ab3335 staining hamster CHO-K1 cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 3% BSA for 1 hour at 37°C.  The primary antibody was diluted 1/200 and incubated with the sample for 12 hours at 4°C.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:

  • Connor-Robson N  et al. An integrated transcriptomics and proteomics analysis reveals functional endocytic dysregulation caused by mutations in LRRK2. Neurobiol Dis 127:512-526 (2019). Read more (PubMed: 30954703) »
  • Spencer B  et al. Reducing Endogenous a-Synuclein Mitigates the Degeneration of Selective Neuronal Populations in an Alzheimer's Disease Transgenic Mouse Model. J Neurosci 36:7971-84 (2016). IHC-FrFl ; Mouse . Read more (PubMed: 27466341) »
See all 9 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Gel Running Conditions
Reduced Denaturing (13%)
Loading amount
20 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Sergi Bayod

Verified customer

Submitted Oct 07 2019

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 05 2015

Application
Western blot
Sample
Human Tissue lysate - whole (brain tissue)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 05 2015

Application
Immunoprecipitation
Immuno-precipitation step
Other - magnetic Dynabeads
Sample
Mouse Tissue lysate - whole (brain)
Specification
brain
Total protein in input
500 µg

Abcam user community

Verified customer

Submitted Feb 10 2015

Application
Western blot
Loading amount
2 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Rat Tissue lysate - whole (hippocampus tissue lysate)
Specification
hippocampus tissue lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C

Abcam user community

Verified customer

Submitted Jun 18 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Human Tissue lysate - whole (BRAIN)
Specification
BRAIN
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Dec 06 2013

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH3T3)
Loading amount
30 µg
Specification
NIH3T3
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 07 2012

Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Loading amount
30 µg
Specification
Hippocampus
Gel Running Conditions
Reduced Denaturing (12% TGS)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Sep 19 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO-K1)
Specification
CHO-K1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Apr 11 2008

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Rat brain)
Specification
Rat brain
Fixative
Paraformaldehyde
Permeabilization
No

Dr. Sophie Pezet

Verified customer

Submitted Aug 28 2007

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