Key features and details
- Rabbit polyclonal to RAB3B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RAB3B antibody
See all RAB3B primary antibodies
DescriptionRabbit polyclonal to RAB3B
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Hamster, Cow
Recombinant full length protein corresponding to Human RAB3B aa 1-219.
MASVTDGKTGVKDASDQNFDYMFKLLIIGNSSVGKTSFLFRYADDTFTPA FVSTVGIDFKVKTVYRHEKRVKLQIWDTAGQERYRTITTAYYRGAMGFIL MYDITNEESFNAVQDWATQIKTYSWDNAQVILVGNKCDMEEERVVPTEKG QLLAEQLGFDFFEASAKENISVRQAFERLVDAICDKMSDSLDTDPSMLGS SKNTRLSDTPPLLQQNCSC
Database link: P20337
- WB: Mouse brain lysate; U-251 MG and U-87 MG whole cell lysates. IHC-P: Human colon cancer tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
sELISA pair antibody
Our Abpromise guarantee covers the use of ab235518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 25 kDa.|
|IHC-P||1/20 - 1/200.|
FunctionProtein transport. Probably involved in vesicular traffic.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationCell membrane.
- Information by UniProt
- Brain antigen RAB3B antibody
- RAB3B antibody
- RAB3B member RAS oncogene family antibody
All lanes : Anti-RAB3B antibody (ab235518) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 25 kDa
Paraffin-embedded human colon cancer tissue stained for RAB3B using ab235518 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235518 has not yet been referenced specifically in any publications.