Overview

  • Product name

    Anti-RAB3B antibody [EPR12987]
    See all RAB3B primary antibodies
  • Description

    Rabbit monoclonal [EPR12987] to RAB3B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RAB3B aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P20337

  • Positive control

    • 293T, HepG2, fetal brain and SW480 lysates; SW480 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    Images

    • Flow cytometric analysis of permeabilized SW480 cells labeling RAB3B with ab177949 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    • All lanes : Anti-RAB3B antibody [EPR12987] (ab177949) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : HepG2 lysate
      Lane 3 : Fetal brain lysate
      Lane 4 : SW480 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 25 kDa

    References

    ab177949 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Oct 11 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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