Recombinant Anti-Rab3D antibody [EPR8106] (ab128997)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)](https://www.abcam.com/ps/products/128/ab128997/Images/ab128997-142885-anti-rab3d-antibody-epr8106-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8106] to Rab3D
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Rab3D antibody [EPR8106]
See all Rab3D primary antibodies -
Description
Rabbit monoclonal [EPR8106] to Rab3D -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Rab3D aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- BxPC-3, T.T, A549, or HT-29 lysate, Human clear cell carcinoma tissue, Human stomach tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.36 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8106 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab128997 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Protein transport. Probably involved in regulated exocytosis. -
Tissue specificity
Highly expressed in granulocytes of peripheral blood. Constitutively expressed at low levels in all hematopoietic cell lines investigated. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9545 Human
- Omim: 604350 Human
- SwissProt: O95716 Human
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Alternative names
- D2-2 antibody
- Glioblastoma overexpressed antibody
- GOV antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)
ab128997, at a 1/250 dilution, staining Human Rab3D in clear cell carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Rab3D antibody [EPR8106] (ab128997)](https://www.abcam.com/ps/products/128/ab128997/Images/ab128997-142886-anti-rab3d-antibody-epr8106-western-blot.jpg)
Western blot - Anti-Rab3D antibody [EPR8106] (ab128997)All lanes : Anti-Rab3D antibody [EPR8106] (ab128997) at 1/10000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : T.T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HT-29 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)](https://www.abcam.com/ps/products/128/ab128997/Images/ab128997-142884-anti-rab3d-antibody-epr8106-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)ab128997, at a 1/250 dilution, staining Human Rab3D in stomach, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Rab3D antibody [EPR8106] (ab128997)](https://www.abcam.com/ps/products/128/ab128997/Images/ab128997-142883-anti-rab3d-antibody-epr8106.jpg)
OI-RD Scanning - Anti-Rab3D antibody [EPR8106] (ab128997) -
![Anti-Rab3D antibody [EPR8106] (ab128997)](https://www.abcam.com/ps/products/128/ab128997/Images/ab128997-5-benefits-of-recombinant-antibodies.png)
Anti-Rab3D antibody [EPR8106] (ab128997)
Protocols
Datasheets and documents
References (3)
ab128997 has been referenced in 3 publications.
- Jin T et al. Lcn2-derived Circular RNA (hsa_circ_0088732) Inhibits Cell Apoptosis and Promotes EMT in Glioma via the miR-661/RAB3D Axis. Front Oncol 10:170 (2020). PubMed: 32154171
- Ren Y et al. MicroRNA-761 is downregulated in colorectal cancer and regulates tumor progression by targeting Rab3D. Exp Ther Med 17:1841-1846 (2019). PubMed: 30783458
- Cao K et al. The lncRNA HOXA11-AS regulates Rab3D expression by sponging miR-125a-5p promoting metastasis of osteosarcoma. Cancer Manag Res 11:4505-4518 (2019). PubMed: 31191012
