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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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RecombinantRabMAb

Recombinant Anti-Rab3D antibody [EPR8106] (ab128997)

  • Datasheet
Reviews (5) Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)
  • Western blot - Anti-Rab3D antibody [EPR8106] (ab128997)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)
  • OI-RD Scanning - Anti-Rab3D antibody [EPR8106] (ab128997)
  • Anti-Rab3D antibody [EPR8106] (ab128997)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8106] to Rab3D
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Recombinant Human Rab3D protein (ab109943)
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View more associated products

Overview

  • Product name

    Anti-Rab3D antibody [EPR8106]
    See all Rab3D primary antibodies
  • Description

    Rabbit monoclonal [EPR8106] to Rab3D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Rab3D aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • BxPC-3, T.T, A549, or HT-29 lysate, Human clear cell carcinoma tissue, Human stomach tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 3.36 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8106
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family

Associated products

  • Alternative Versions

    • Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HT29 whole cell lysate (ab3952)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human Rab3D protein (ab109943)

Applications

Our Abpromise guarantee covers the use of ab128997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Protein transport. Probably involved in regulated exocytosis.
    • Tissue specificity

      Highly expressed in granulocytes of peripheral blood. Constitutively expressed at low levels in all hematopoietic cell lines investigated.
    • Sequence similarities

      Belongs to the small GTPase superfamily. Rab family.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession O95716 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9545 Human
      • Omim: 604350 Human
      • SwissProt: O95716 Human
      • Alternative names

        • D2-2 antibody
        • Glioblastoma overexpressed antibody
        • GOV antibody
        • RAB 16 antibody
        • RAB16 antibody
        • Rab3d antibody
        • Rab3D upregulated with myeloid differentiation antibody
        • RAB3D, member RAS oncogene family antibody
        • RAB3D_HUMAN antibody
        • RAD3D antibody
        • Ras-related protein Rab-3D antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)

        ab128997, at a 1/250 dilution, staining Human Rab3D in clear cell carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Western blot - Anti-Rab3D antibody [EPR8106] (ab128997)
        Western blot - Anti-Rab3D antibody [EPR8106] (ab128997)
        All lanes : Anti-Rab3D antibody [EPR8106] (ab128997) at 1/10000 dilution

        Lane 1 : BxPC-3 cell lysate
        Lane 2 : T.T cell lysate
        Lane 3 : A549 cell lysate
        Lane 4 : HT-29 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 25 kDa
        Observed band size: 25 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)

        ab128997, at a 1/250 dilution, staining Human Rab3D in stomach, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • OI-RD Scanning - Anti-Rab3D antibody [EPR8106] (ab128997)
        OI-RD Scanning - Anti-Rab3D antibody [EPR8106] (ab128997)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-Rab3D antibody [EPR8106] (ab128997)
        Anti-Rab3D antibody [EPR8106] (ab128997)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (3)

      Publishing research using ab128997? Please let us know so that we can cite the reference in this datasheet.

      ab128997 has been referenced in 3 publications.

      • Jin T  et al. Lcn2-derived Circular RNA (hsa_circ_0088732) Inhibits Cell Apoptosis and Promotes EMT in Glioma via the miR-661/RAB3D Axis. Front Oncol 10:170 (2020). PubMed: 32154171
      • Ren Y  et al. MicroRNA-761 is downregulated in colorectal cancer and regulates tumor progression by targeting Rab3D. Exp Ther Med 17:1841-1846 (2019). PubMed: 30783458
      • Cao K  et al. The lncRNA HOXA11-AS regulates Rab3D expression by sponging miR-125a-5p promoting metastasis of osteosarcoma. Cancer Manag Res 11:4505-4518 (2019). PubMed: 31191012

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-5 of 5 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab3D antibody [EPR8106]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Mice hepatocytes)
      Permeabilization
      Yes - 0.2% Triton X-100 in PBS
      Specification
      Mice hepatocytes
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Dr. Armen Petrosyan

      Verified customer

      Submitted May 24 2018

      Western blot abreview for Anti-Rab3D antibody [EPR8106]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - other (Mice hepatocytes - plasma membrane fraction)
      Gel Running Conditions
      Reduced Denaturing (4-15% SDS PAGE)
      Loading amount
      25 µg
      Specification
      Mice hepatocytes - plasma membrane fraction
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Dr. Armen Petrosyan

      Verified customer

      Submitted Apr 25 2018

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab3D antibody [EPR8106]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (Rat hepatocytes)
      Permeabilization
      Yes - 0.2% Triton X-100
      Specification
      Rat hepatocytes
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Dr. Armen Petrosyan

      Verified customer

      Submitted Nov 30 2016

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab3D antibody [EPR8106]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (WIF-B cells, the hepatoma-derived hybrid cell line)
      Permeabilization
      Yes - 0.2% Triton X-100
      Specification
      WIF-B cells, the hepatoma-derived hybrid cell line
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Dr. Armen Petrosyan

      Verified customer

      Submitted Oct 31 2016

      Western blot abreview for Anti-Rab3D antibody [EPR8106]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HepG2 cells)
      Gel Running Conditions
      Reduced Denaturing (12% SDS-PAGE)
      Loading amount
      20 µg
      Specification
      HepG2 cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Dr. Armen Petrosyan

      Verified customer

      Submitted Oct 27 2016

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