Name: Anti-Rab3D antibody [EPR8106]
Brand: RabMAb
SKU: ab128997
Rating: 5 (5 reviews)

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Protein

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Primary - Rabbit Anti-Rab3D antibody [EPR8106] (ab128997)
WB, IHC-P

Protein - Recombinant Human Rab3D protein (ab109943)
SDS-PAGE, MS

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Product name

Anti-Rab3D antibody [EPR8106]
See all Rab3D primary antibodies
Description

Rabbit monoclonal [EPR8106] to Rab3D
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human Rab3D aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- BxPC-3, T.T, A549, or HT-29 lysate, Human clear cell carcinoma tissue, Human stomach tissue.
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 3.36 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8106
Isotype

IgG
Research areas

Alternative Versions
- Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HT29 whole cell lysate (ab3952)
- A549 whole cell lysate (ab7910)
Recombinant Protein
- Recombinant Human Rab3D protein (ab109943)

Our Abpromise guarantee covers the use of ab128997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt,ICC or IP.

Function

Protein transport. Probably involved in regulated exocytosis.
Tissue specificity

Highly expressed in granulocytes of peripheral blood. Constitutively expressed at low levels in all hematopoietic cell lines investigated.
Sequence similarities

Belongs to the small GTPase superfamily. Rab family.
Cellular localization

Cell membrane.
Target information above from: UniProt accession O95716 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 9545 Human
- Omim: 604350 Human
- SwissProt: O95716 Human
Alternative names
- D2-2 antibody
- Glioblastoma overexpressed antibody
- GOV antibody
- RAB 16 antibody
- RAB16 antibody
- Rab3d antibody
- Rab3D upregulated with myeloid differentiation antibody
- RAB3D, member RAS oncogene family antibody
- RAB3D_HUMAN antibody
- RAD3D antibody
- Ras-related protein Rab-3D antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)

ab128997, at a 1/250 dilution, staining Human Rab3D in clear cell carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Rab3D antibody [EPR8106] (ab128997)

All lanes : Anti-Rab3D antibody [EPR8106] (ab128997) at 1/10000 dilution

Lane 1 : BxPC-3 cell lysate
Lane 2 : T.T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HT-29 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : 'standard' HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)

ab128997, at a 1/250 dilution, staining Human Rab3D in stomach, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Other - Anti-Rab3D antibody [EPR8106] (ab128997)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D

Western blot protocols
Immunohistochemistry protocols

Datasheet

This product has been referenced in:

Ren Y et al. MicroRNA-761 is downregulated in colorectal cancer and regulates tumor progression by targeting Rab3D. Exp Ther Med 17:1841-1846 (2019). Read more (PubMed: 30783458) »
Cao K et al. The lncRNA HOXA11-AS regulates Rab3D expression by sponging miR-125a-5p promoting metastasis of osteosarcoma. Cancer Manag Res 11:4505-4518 (2019). Read more (PubMed: 31191012) »

See all 2 Publications for this product

Publishing research using ab128997? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Mice hepatocytes)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

Mice hepatocytes

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted May 24 2018

Application

Western blot

Sample

Mouse Tissue lysate - other (Mice hepatocytes - plasma membrane fraction)

Gel Running Conditions

Reduced Denaturing (4-15% SDS PAGE)

Loading amount

25 µg

Specification

Mice hepatocytes - plasma membrane fraction

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Apr 25 2018

Application

Immunocytochemistry/ Immunofluorescence

Sample

Rat Cell (Rat hepatocytes)

Permeabilization

Yes - 0.2% Triton X-100

Specification

Rat hepatocytes

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Nov 30 2016

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (WIF-B cells, the hepatoma-derived hybrid cell line)

Permeabilization

Yes - 0.2% Triton X-100

Specification

WIF-B cells, the hepatoma-derived hybrid cell line

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Oct 31 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (HepG2 cells)

Gel Running Conditions

Reduced Denaturing (12% SDS-PAGE)

Loading amount

20 µg

Specification

HepG2 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Oct 27 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab3D antibody [EPR8106]

Western blot abreview for Anti-Rab3D antibody [EPR8106]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab3D antibody [EPR8106]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab3D antibody [EPR8106]

Western blot abreview for Anti-Rab3D antibody [EPR8106]

Dissociation constant (K_D)