Recombinant Anti-Rab3D antibody [EPR8106] - BSA and Azide free (ab248251)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8106] to Rab3D - BSA and Azide free
- Suitable for: IHC-P, mIHC, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Rab3D antibody [EPR8106] - BSA and Azide free
See all Rab3D primary antibodies -
Description
Rabbit monoclonal [EPR8106] to Rab3D - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, mIHC, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- BxPC-3, T.T, A549, or HT-29 lysate, Human clear cell carcinoma tissue, Human stomach tissue. mIHC: Human stomach tissue.
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General notes
ab248251 is the carrier-free version of ab128997.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 3.36 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8106 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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mIHC |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
mIHC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
Target
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Function
Protein transport. Probably involved in regulated exocytosis. -
Tissue specificity
Highly expressed in granulocytes of peripheral blood. Constitutively expressed at low levels in all hematopoietic cell lines investigated. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9545 Human
- Omim: 604350 Human
- SwissProt: O95716 Human
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Alternative names
- D2-2 antibody
- Glioblastoma overexpressed antibody
- GOV antibody
see all
Images
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Fluorescence multiplex immunohistochemical analysis of the human stomach (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Chromogranin A (ab254557, gray; Opal™690), anti-MUC-6 (ab223846, green; Opal™520) and anti-Rab3D (ab128997, red; Opal™570) on human stomach. Panel B: anti-MUC-6 stained on mucous neck cells. Panel C: anti-Chromogranin A stained on neuroendocrine cells. Panel D: anti-Rab3D stained on Chief cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab254557 (1/5000), ab223846 (1/1000), and ab128997 (1/10000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
This data was developed using ab128997, the same antibody clone in a different buffer formulation.
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Fluorescence multiplex immunohistochemical analysis of the human stomach (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Mucin 5AC (ab3649, gray; Opal™690), anti-MUC-6 (ab223846, green; Opal™520) and anti-Rab3D (ab128997, red; Opal™570) on human stomach. Panel B: anti-MUC-6 stained on mucous neck cells. Panel C: anti-Mucin 5AC stained on surface mucous cells. Panel D: anti-Rab3D stained on Chief cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab3649 (1/5000 dilution), ab223846 (1/1000 dilution), and ab128997 (1/10000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
This data was developed using ab128997, the same antibody clone in a different buffer formulation.
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This data was developed using ab128997, the same antibody clone in a different buffer formulation.ab128997, at a 1/250 dilution, staining Human Rab3D in clear cell carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Rab3D antibody [EPR8106] (ab128997) at 1/10000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : T.T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HT-29 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaThis data was developed using ab128997, the same antibody clone in a different buffer formulation.
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This data was developed using ab128997, the same antibody clone in a different buffer formulation.ab128997, at a 1/250 dilution, staining Human Rab3D in stomach, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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This data was developed using ab128997, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248251 has not yet been referenced specifically in any publications.