Key features and details
- Rabbit polyclonal to RAB3GAP1
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RAB3GAP1 antibody
See all RAB3GAP1 primary antibodies
DescriptionRabbit polyclonal to RAB3GAP1
Specificityab74447 detects endogenous levels of total RAB3GAP1 protein. Does not react with mouse samples, by honology and testing for NIH3T3
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Does not react with: Mouse
Synthetic peptide derived from an internal region of human RAB3GAP1.
- Human testis tissue; 293 cell extracts.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab74447 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab74447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 111 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionProbable catalytic subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Specifically converts active Rab3-GTP to the inactive form Rab3-GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters.
Involvement in diseaseDefects in RAB3GAP1 are the cause of Warburg micro syndrome type 1 (WARBM1) [MIM:600118]. A rare syndrome characterized by microcephaly, microphthalmia, microcornia, congenital cataracts, optic atrophy, cortical dysplasia, in particular corpus callosum hypoplasia, severe mental retardation, spastic diplegia, and hypogonadism.
Sequence similaritiesBelongs to the Rab3-GAP catalytic subunit family.
Cellular localizationCytoplasm. In neurons, it is enriched in the synaptic soluble fraction.
- Information by UniProt
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ab74447, at a 1/50 dilution, staining RAB3GAP1 in paraffin embedded human testis tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
All lanes : Anti-RAB3GAP1 antibody (ab74447) at 1/500 dilution
Lane 1 : 293 cell extracts
Lane 2 : 293 cell extracts with immunizing peptide at 10 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 111 kDa
Observed band size: 111 kDa
ab74447 has not yet been referenced specifically in any publications.