Anti-RAB3GAP1 antibody - C-terminal (ab229589)
Key features and details
- Rabbit polyclonal to RAB3GAP1 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RAB3GAP1 antibody - C-terminal -
Description
Rabbit polyclonal to RAB3GAP1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Xenopus laevis -
Immunogen
Recombinant fragment within Human RAB3GAP1 (C terminal). The exact sequence is proprietary.
Database link: Q15042 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000.
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Notes |
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WB
1/500 - 1/3000. |
Target
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Function
Probable catalytic subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Specifically converts active Rab3-GTP to the inactive form Rab3-GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in RAB3GAP1 are the cause of Warburg micro syndrome type 1 (WARBM1) [MIM:600118]. A rare syndrome characterized by microcephaly, microphthalmia, microcornia, congenital cataracts, optic atrophy, cortical dysplasia, in particular corpus callosum hypoplasia, severe mental retardation, spastic diplegia, and hypogonadism. -
Sequence similarities
Belongs to the Rab3-GAP catalytic subunit family. -
Cellular localization
Cytoplasm. In neurons, it is enriched in the synaptic soluble fraction. - Information by UniProt
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Database links
- Entrez Gene: 22930 Human
- Entrez Gene: 226407 Mouse
- Entrez Gene: 447515 Xenopus laevis
- Omim: 602536 Human
- SwissProt: Q15042 Human
- SwissProt: Q80UJ7 Mouse
- SwissProt: Q642R9 Xenopus laevis
- Unigene: 306327 Human
see all -
Alternative names
- DKFZp434A012 antibody
- KIAA0066 antibody
- P130 antibody
see all
Images
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All lanes : Anti-RAB3GAP1 antibody - C-terminal (ab229589) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.7.5% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229589 has not yet been referenced specifically in any publications.