Anti-RAB3GAP1 antibody - C-terminal (ab229589)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RAB3GAP1 antibody - C-terminal
See all RAB3GAP1 primary antibodies -
Description
Rabbit polyclonal to RAB3GAP1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Xenopus laevis
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Immunogen
Recombinant fragment within Human RAB3GAP1 (C terminal). The exact sequence is proprietary.
Database link: Q15042 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab229589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. |
Target
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Function
Probable catalytic subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Specifically converts active Rab3-GTP to the inactive form Rab3-GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in RAB3GAP1 are the cause of Warburg micro syndrome type 1 (WARBM1) [MIM:600118]. A rare syndrome characterized by microcephaly, microphthalmia, microcornia, congenital cataracts, optic atrophy, cortical dysplasia, in particular corpus callosum hypoplasia, severe mental retardation, spastic diplegia, and hypogonadism. -
Sequence similarities
Belongs to the Rab3-GAP catalytic subunit family. -
Cellular localization
Cytoplasm. In neurons, it is enriched in the synaptic soluble fraction. - Information by UniProt
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Database links
- Entrez Gene: 22930 Human
- Entrez Gene: 226407 Mouse
- Entrez Gene: 447515 Xenopus laevis
- Omim: 602536 Human
- SwissProt: Q15042 Human
- SwissProt: Q80UJ7 Mouse
- SwissProt: Q642R9 Xenopus laevis
- Unigene: 306327 Human
see all -
Alternative names
- DKFZp434A012 antibody
- KIAA0066 antibody
- P130 antibody
see all
Images
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Western blot - Anti-RAB3GAP1 antibody - C-terminal (ab229589)All lanes : Anti-RAB3GAP1 antibody - C-terminal (ab229589) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.7.5% SDS-PAGE gel.
References
ab229589 has not yet been referenced specifically in any publications.
