Anti-RAB3GAP2 antibody (ab110462)
Key features and details
- Rabbit polyclonal to RAB3GAP2
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
-
Product name
Anti-RAB3GAP2 antibody -
Description
Rabbit polyclonal to RAB3GAP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide derived from an internal region of Human RAB3GAP2 (UniProt ID: Q9H2M9).
-
Positive control
- Human breast carcinoma tissue; extracts from COS cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab110462 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 156 kDa).
|
|
IHC-P |
1/50 - 1/100. Antigen retrieval is heat-mediated, citrate buffer pH 6.0. A pressure cooker was used for about 5 minutes.
|
Notes |
---|
WB
1/500 - 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 156 kDa). |
IHC-P
1/50 - 1/100. Antigen retrieval is heat-mediated, citrate buffer pH 6.0. A pressure cooker was used for about 5 minutes. |
Target
-
Relevance
RAB3GAP2 is a regulatory subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Rab3 GTPase-activating complex specifically converts active Rab3-GTP to the inactive form Rab3-GDP. It is required for normal eye and brain development and may participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters. -
Cellular localization
Cytoplasmic. Note: In neurons, it is enriched in the synaptic soluble fraction. -
Database links
- Entrez Gene: 25782 Human
- Entrez Gene: 98732 Mouse
- Entrez Gene: 289350 Rat
- Omim: 609275 Human
- SwissProt: Q9H2M9 Human
- SwissProt: Q8BMG7 Mouse
- SwissProt: Q5U1Z0 Rat
-
Alternative names
- DKFZp434D245 antibody
- FLJ14579 antibody
- KIAA0839 antibody
see all
Images
-
All lanes : Anti-RAB3GAP2 antibody (ab110462) at 1/500 dilution
Lane 1 : Extracts from COS cells
Lane 2 : Extracts from COS cells, treated with the synthesized peptide
Lysates/proteins at 30 µg per lane.
Predicted band size: 156 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted? -
ab110462, at a 1/50 dilution, staining RAB3GAP2 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the synthesized peptide.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab110462 has not yet been referenced specifically in any publications.