Key features and details
- Rabbit polyclonal to RAB3GAP2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RAB3GAP2 antibody
See all RAB3GAP2 primary antibodies
DescriptionRabbit polyclonal to RAB3GAP2
Specificityab110462 detects endogenous levels of total RAB3GAP2 protein.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from an internal region of Human RAB3GAP2 (UniProt ID: Q9H2M9).
- Human breast carcinoma tissue; extracts from COS cells
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab110462 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab110462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 156 kDa).|
|IHC-P||1/50 - 1/100. Antigen retrieval is heat-mediated, citrate buffer pH 6.0. A pressure cooker was used for about 5 minutes.|
RelevanceRAB3GAP2 is a regulatory subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Rab3 GTPase-activating complex specifically converts active Rab3-GTP to the inactive form Rab3-GDP. It is required for normal eye and brain development and may participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters.
Cellular localizationCytoplasmic. Note: In neurons, it is enriched in the synaptic soluble fraction.
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All lanes : Anti-RAB3GAP2 antibody (ab110462) at 1/500 dilution
Lane 1 : Extracts from COS cells
Lane 2 : Extracts from COS cells, treated with the synthesized peptide
Lysates/proteins at 30 µg per lane.
Predicted band size: 156 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted?
ab110462, at a 1/50 dilution, staining RAB3GAP2 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the synthesized peptide.
ab110462 has not yet been referenced specifically in any publications.