Overview

  • Product name
  • Description
    Rabbit polyclonal to Rab4
  • Host species
    Rabbit
  • Specificity
    This antibody is very specific for Rab4. To our knowledge this antibody does not cross-react with other Rab proteins. However, as there are dozens of different proteins in the Rab family, not every single one has been tested for cross-reactivity with this antibody.
  • Tested applications
    Suitable for: ICC/IF, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Recombinant fusion protein (Human) expressed in E.coli.

  • Positive control
    • Mouse or Rat Brain Tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab13252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 17547697
WB 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 25.6 kDa).
ICC 1/200 - 1/500.

Target

Images

  • ab13252 Rabbit polyclonal to Rab4 was used in fixed primary cultures of rat hippocampal neurons (embryonic day 18) at a dilution of 1/200. A secondary goat anti-rabbit antibody was used for detection (Alexa Fluor 488; 1/200; green). Confocal microscopy revealed typically punctuate immunofluorescence expected of Rab4 in these neurons. The red colour labelling is that of monoclonal mouse anti-

    Anti-Rab4 antibody (ab13252) at 1/1000 dilution + Lysates prepared from rat brain tissue

    Predicted band size: 25.6 kDa

References

This product has been referenced in:
  • Do MT  et al. Isoprenylcysteine carboxylmethyltransferase function is essential for RAB4A-mediated integrin ß3 recycling, cell migration and cancer metastasis. Oncogene 36:5757-5767 (2017). WB, IP ; Human . Read more (PubMed: 28604748) »
  • Tsai SY  et al. Regulation of TLR3 Activation by S100A9. J Immunol 195:4426-37 (2015). Read more (PubMed: 26385519) »
See all 18 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HRMEC)
Permeabilization
Yes - 0.1% Triton in PBS
Specification
HRMEC
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 23 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS7)
Permeabilization
Yes - 0.2%Triton
Specification
COS7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 07 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (bEnd.3)
Permeabilization
Yes - 0.1% Triton in PBS%
Specification
bEnd.3
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 03 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T cells)
Loading amount
25 µg
Specification
HEK293T cells
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 28 2013

Answer

The antibody was raised against the whole molecule of human Rab4 and was expressed in E.coli.

Read More

Question
Answer

Thank you for the further information. It is difficult to select alternatives that are the most 'specific' as we have not tested these antibodies side by side in the same sample application or species. Reviewing the datasheets I think that ab74906 anti-Rab7 antibody and ab3612 anti-Rabb11 antibody may be preferable but please do check the datasheets to confirm whether you agree. Please bear in mind that ab74906 (rabbit polyclonal to Rab7) is not tested in ICC, and as it shows a weak band in WB the staining intensity may be lower than other antibodies. In addition, ab3612 (rabbit polyclonal to Rab11) is only predicted to work in mouse samples, and may not give positive results. As the Rab7 and Rab11 antibodies will be used in previously untested species/applications, would it be possible to submit an Abreview with your results? It doesn't matter if it is positive or negative, but the information will be really useful for other researchers that may be interested in using these products in a similar manner. I will be able to provide ab78970 and ab87802. Could you also submit Abreview information for these products too? Any other information you be able to share via our Abreview system would be extremely useful. Looking forward to your reply.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (Bovine Brain Microvascular Endothelial Cells)
Specification
Bovine Brain Microvascular Endothelial Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Saponin 0.1%
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C

Mr. Cyril Mougin

Verified customer

Submitted Sep 07 2010

Answer

To our knowledge, this antibody does not cross-react with other Rab proteins. However, as there are dozens of different proteins in the Rab family, not every single one has been tested for cross-reactivity with this antibody. To the best of our knowledge this antibody is very specific for Rab4. Please contact us again if you have any additional questions.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cell (Hippocampal neuron culture)
Specification
Hippocampal neuron culture
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 5%

Dr. Randal Moldrich

Verified customer

Submitted Dec 07 2005

Answer

The antibody ab13252 is specific for Rab4, a 25 kDa GTP-binding protein that regulates recycling of proteins from the early endosomes to the cell surface and therefore will not recognise the whole endosome, I hope this information helps,

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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