Key features and details
- Rabbit polyclonal to Rab4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Rab4 antibody
See all Rab4 primary antibodies
DescriptionRabbit polyclonal to Rab4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Cow, Zebrafish, Rhesus monkey
Recombinant fragment within Human Rab4 (internal sequence). The exact sequence is proprietary.
Database link: P20338
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysate. IHC-P: Human lung carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228545 in the following tested applications.
|WB||1/500 - 1/3000. Predicted molecular weight: 24 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionProtein transport. Probably involved in vesicular traffic.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
modificationsPhosphorylated by CDK1 kinase during mitosis.
Cellular localizationMembrane. Cytoplasm. Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1.
- Information by UniProt
- HRES 1 / RAB4 antibody
- Oncogene RAB4 antibody
- Rab 4 antibody
All lanes : Anti-Rab4 antibody (ab228545) at 1/200 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Paraffin-embedded human lung carcinoma tissue stained for Rab4 using ab228545 at 1/250 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228545 has not yet been referenced specifically in any publications.