Overview

  • Product name

  • Description

    Rabbit polyclonal to Rab4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Rab4 (internal sequence). The exact sequence is proprietary.
    Database link: P20338

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell lysate. IHC-P: Human lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab228545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-Rab4 antibody (ab228545) at 1/200 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa



    12% SDS-PAGE

  • Paraffin-embedded human lung carcinoma tissue stained for Rab4 using ab228545 at 1/250 dilution in immunohistochemical analysis.

References

ab228545 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228545.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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