Key features and details
- Rabbit polyclonal to Rab4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Rab4 antibody
See all Rab4 primary antibodies
DescriptionRabbit polyclonal to Rab4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Rab4 (C terminal).
Database link: P20338
- IHC-P: Human pancreas and liver tissues. WB: HeLa cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferWhole antiserum.
Concentration information loading...
Our Abpromise guarantee covers the use of ab230301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).|
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionProtein transport. Probably involved in vesicular traffic.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
modificationsPhosphorylated by CDK1 kinase during mitosis.
Cellular localizationMembrane. Cytoplasm. Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1.
- Information by UniProt
- HRES 1 / RAB4 antibody
- Oncogene RAB4 antibody
- Rab 4 antibody
Formalin-fixed, paraffin-embedded human pancreas tissue stained for Rab4 using ab230301 at 1/200 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver tissue stained for Rab4 using ab230301 at 1/200 dilution in immunohistochemical analysis.
Anti-Rab4 antibody (ab230301) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 24 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230301 has not yet been referenced specifically in any publications.