Validated using a knockout cell line

Recombinant Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab108974)


  • Product name

    Anti-Rab4 antibody [EPR3042] - Early Endosome Marker
    See all Rab4 primary antibodies
  • Description

    Rabbit monoclonal [EPR3042] to Rab4 - Early Endosome Marker
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Rab4 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • MCF7, PC12, Neuro-2a, mouse brain, rat brain and fetal brain cell lysates
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab108974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target


    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Rab4 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human fetal brain cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108974 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

      Unpurified ab108974 was shown to recognize Rab4 when Rab4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Rab4 knockout samples were subjected to SDS-PAGE. Unpurified ab108974 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab108974) at 1/2000 dilution (purified)

      Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
      Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
      Lane 4 : Mouse brain lysates
      Lane 5 : Rat brain lysates

      Lysates/proteins at 20 µg per lane.

      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 24 kDa
      Observed band size: 25 kDa
      why is the actual band size different from the predicted?

      Blocking and diluting buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Rab4 with purified ab108974 at 1:100 dilution (8.8μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunofluorescent staining of Rab4 in HeLa cells, using unpurified ab108974 at a 1/100 dilution.

    • All lanes : Anti-Rab4 antibody [EPR3042] - Early Endosome Marker (ab108974) at 1/2000 dilution (unpurified)

      Lane 1 : MCF7 cell lysates
      Lane 2 : PC12 cell lysates
      Lane 3 : Fetal brain cell lysates

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 24 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?


    This product has been referenced in:

    • Faraz M  et al. A protein interaction network centered on leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) regulates growth factor receptors. J Biol Chem 293:3421-3435 (2018). Read more (PubMed: 29317492) »
    • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). Read more (PubMed: 30377371) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A


    Deux anticorps semblent intéressants pour la détection de Rab4 chez la Drosophile:
    Le premier est un polyclonal de lapin dont l'immunogène présente 86% d'homologie avec la protéine d'intéret (voir fichier pdf ci-joint "ab78970 vs Drosophila"). La référence de cet anticorps est: ab78970 et le lien vers la fiche technique est
    Le second est un RabMAb, ou monoclonal de lapin, dont l'immunogène présente 71% d'homologie avec la protéine d'intéret (voir fichier pdf ci-joint "ab108974 vs Drosophila"). La référence de cet anticorps est: ab108974 et le lien vers la fiche technique est
    Nous avons en ce moment une promotion qui vous donne droit à un monoclonal de lapin gratuit pour l'achat d'un autre anticorps.
    De plus, je peux vous offrir une offre de test pour le ab78970. En voici les détails:
    1. Nous confirmer que vous souhaitez tester ab78970 chez la drosophile afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab78970
    2. Acheter ab78970 par téléphone, fax ou internet (
    3. Le tester chez la drosophile
    4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations
    5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
    Même si ab78970 ne pas fonctionne pas chez la drosophile, après soumission de votre Abreview, vous recevrez quand même un avoir de la valeur de l'anticorps testé.
    Termes et Conditions :
    Merci de m'indiquer si vous souhaitez bénéficier de cette offre de test pour le ab78970. En attendant votre réponse je vous envoie dans un autre email un devis pour le ab78970 et le ab108974.

    Read More


    Thank you for getting back to me with that information.

    I am sorry, I did miss understand you, I thought you were using tissue, not cell lines. This does make the band at 50 kDa unexpected, although I wouldsay that a"no-primary" control would still be worth performing.

    I am sorry to hear that two of our other antibodies have not been performing as expected either. Could you please provide information as to what results you have had with these antibodies? I would really like to look into this further in order to understand what may be going wrong. If possible, could you fill out the questionnaire I have attached to this email, adding any images that you have of the blots would also be very useful.

    Could you also please provide mewith the catalogue numbers and lot numbers of the antibodies used? If youdo not have access to this then the order numbers?Which is the new monoclonal you have purchased from us?

    May I alsoask, have you used any other anti-Rab4 antibodies from any other suppliers? What have been the results of this?

    I look forward to receiving your reply.

    Read More


    Thank you for the swift reply.

    The extra details have made it much easier to understand the problems you have been encountering. Although there is a faint band at the expected size (˜25 kDa), as you have described, the non-specific band at ˜45-50 kDa is much stronger. I think this band may possible be from the rat IgG being picked up but the goat anti-rabbit secondary antibody. Could you giveme further detailsof the secondary antibody used? Has this secondary been used with rat samples previously? I would suggest thatit may be worthwhile toperforma no-primary control and/or use adifferent anti-rabbit antibodyin order to investigate this.

    The blot produced, is itthe first time ithas been probed or has a different primary antibody beendetected using the same blot followed by stripping? If so couldyou describe how this was performed?

    Many thanks for your continued cooperation.

    Read More


    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with the anti-Rab4 antibody (ab108974). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    Thank you forproviding the details of the experiment you have performed. This information will allow us to investigate this case internally and initiate additional testing where necessary. In order to understand the situation more fully, and hopefully help to improve the results obtained so far, could you please provide the following additional information:

    1. What lysateshave been used with the antibody and how have these been prepared prior to loading on the gel? - how was the sample lysed (lysis buffer, protease inhibitors?) and was the sample denatured by boiling and a reducing agent used?

    2. Was any loading control preformed in order to ascertain the quality of the samples? I.e. detection of something like actin or tubulin?

    3. Has the secondary antibody been used successfully before in Western blotting with any other primary antibodies?

    4. If you would be able to send an image of the blot obtained this would be very helpful in understanding what may be contributing to the unexpected results.

    5. Would you be able to tell be the order number from which you ordered this antibody?

    If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

    I look forward to receiving your reply.

    Read More

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