Product nameAnti-RAB40B antibody
DescriptionRabbit polyclonal to RAB40B
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
- 293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab82896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/12500.
RelevanceRAB40B is similar to a yeast protein which suggests a role of the gene product in regulating secretory vesicles. It may be a substrate recognition component of a SCF like ECS (Elongin Cullin SOCS box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Cellular localizationCell membrane; Lipid anchor; Cytoplasmic side
- FLJ42385 antibody
- GTP binding protein homologous to Saccharomyces cerevisiae SEC4 antibody
- RAB40B member RAS oncogene family antibody
Anti-RAB40B antibody (ab82896) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
ab82896 has not yet been referenced specifically in any publications.