Anti-RAB4B antibody - N-terminal (ab179948)

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Western blot - Anti-RAB4B antibody - N-terminal (ab179948)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB4B antibody - N-terminal (ab179948)

Key features and details

  • Rabbit polyclonal to RAB4B - N-terminal
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RAB4B antibody - N-terminal

  • Description

    Rabbit polyclonal to RAB4B - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Zebrafish

  • Immunogen

    Synthetic peptide within Human RAB4B aa 29-58 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    IENKFKQDSNHTIGVEFGSRVVNVGGKTVK


    Database link: P61018
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • CEM cell lysate; Human kidney tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml.
WB
1/100 - 1/500. Predicted molecular weight: 24 kDa.
Notes
IHC-P
Use a concentration of 5 µg/ml.
WB
1/100 - 1/500. Predicted molecular weight: 24 kDa.

Target

Images

  • Western blot - Anti-RAB4B antibody - N-terminal (ab179948)
    Western blot - Anti-RAB4B antibody - N-terminal (ab179948)
    Anti-RAB4B antibody - N-terminal (ab179948) at 1/100 dilution + CEM cell lysate at 35 µg

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB4B antibody - N-terminal (ab179948)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB4B antibody - N-terminal (ab179948)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling RAB4B with ab179948 at 5 µg/ml.

References (0)

Publishing research using ab179948? Please let us know so that we can cite the reference in this datasheet.

ab179948 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-RAB4B antibody - N-terminal

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Neural Precursor)
Permeabilization
Yes - 0.01% Triton X-100
Specification
Neural Precursor
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Verified customer

Submitted Oct 26 2016

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