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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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Anti-RAB4B antibody - N-terminal (ab179948)

  • Datasheet
  • SDS
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Western blot - Anti-RAB4B antibody - N-terminal (ab179948)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB4B antibody - N-terminal (ab179948)

Key features and details

  • Rabbit polyclonal to RAB4B - N-terminal
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Recombinant human RAB4B protein (ab90048)

View more associated products

Overview

  • Product name

    Anti-RAB4B antibody - N-terminal
  • Description

    Rabbit polyclonal to RAB4B - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide within Human RAB4B aa 29-58 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    IENKFKQDSNHTIGVEFGSRVVNVGGKTVK


    Database link: P61018
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • CEM cell lysate; Human kidney tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human RAB4B protein (ab90048)
  • Related Products

    • Recombinant human RAB4B protein (ab90048)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml.
WB
1/100 - 1/500. Predicted molecular weight: 24 kDa.
Notes
IHC-P
Use a concentration of 5 µg/ml.
WB
1/100 - 1/500. Predicted molecular weight: 24 kDa.

Target

  • Function

    Protein transport. Probably involved in vesicular traffic.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P61018 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 403956 Dog
    • Entrez Gene: 53916 Human
    • Entrez Gene: 19342 Mouse
    • Entrez Gene: 50866 Rat
    • Entrez Gene: 445498 Zebrafish
    • Omim: 612945 Human
    • SwissProt: P61017 Dog
    • SwissProt: P61018 Human
    • SwissProt: Q91ZR1 Mouse
    • SwissProt: P51146 Rat
    • SwissProt: Q68EK7 Zebrafish
    • Unigene: 631539 Human
    • Unigene: 730737 Human
    • Unigene: 482114 Mouse
    • Unigene: 7181 Rat
    • Unigene: 85594 Zebrafish
    see all
  • Alternative names

    • 1500031G17Rik antibody
    • FLJ78649 antibody
    • MGC52123 antibody
    • OTTMUSP00000026157 antibody
    • RAB4B antibody
    • RAB4B, member RAS oncogene family antibody
    • RAB4B_HUMAN antibody
    • Ras related GTP binding protein 4b antibody
    • Ras related protein Rab 4B antibody
    • Ras-related protein Rab-4B antibody
    • Small GTP binding protein RAB4B antibody
    see all

Images

  • Western blot - Anti-RAB4B antibody - N-terminal (ab179948)
    Western blot - Anti-RAB4B antibody - N-terminal (ab179948)
    Anti-RAB4B antibody - N-terminal (ab179948) at 1/100 dilution + CEM cell lysate at 35 µg

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB4B antibody - N-terminal (ab179948)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB4B antibody - N-terminal (ab179948)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling RAB4B with ab179948 at 5 µg/ml.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab179948? Please let us know so that we can cite the reference in this datasheet.

ab179948 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-RAB4B antibody - N-terminal

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Neural Precursor)
Permeabilization
Yes - 0.01% Triton X-100
Specification
Neural Precursor
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

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