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Our Abpromise guarantee covers the use of ab190215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 24 kDa.|
|IHC-P||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/10 - 1/50.|
Immunohistochemical analysis of formalin fixed, paraffin embedded Human prostate tissue labeling Rab5 with ab190215 at 1/50 dilution.
Immunofluorescence on paraffin-embedded Human brain tissue labeling Rab5 with ab190215 at 25 μg/ml, followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.
Flow cytometry of HL60 cells (right histogram) compared to a negative control cell (left histogram), labeling Rab5 with ab190215 at 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab190215 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"