Overview

  • Product name

    Anti-Rab5 antibody - C-terminal
    See all Rab5 primary antibodies
  • Description

    Rabbit polyclonal to Rab5 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human Rab5 (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: P20339

  • Positive control

    • WB: A431 and Raji whole cell lysate; Mouse brain lysate. IP: HeLa whole cell lysate. IHC-P: U-87 MG xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab228770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-Rab5 antibody - C-terminal (ab228770) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 24 kDa



    12% SDS-PAGE gel.

  • Rab5 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab228770. Western blot was performed from the immunoprecipitate using ab228770 at 1/1000 dilution. Anti-rabbit IgG was used as secondary antibody.

    Lane 1: HeLa whole cell lysate 30 µg (Input).

    Lane 2: Control IP with 2 μg of preimmune rabbit IgG in HeLa whole cell lysate.

    Lane 3: ab228770 IP in HeLa whole cell lysate. 

    15% SDS-PAGE gel.

     

  • Anti-Rab5 antibody - C-terminal (ab228770) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 24 kDa

  • Paraffin-embedded U-87 MG (human glioblastoma-astrocytoma epithelial cell line) xenograft stained for Rab5 using ab228770 at 1/500 dilution in immunohistochemical analysis.

References

ab228770 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228770.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up