Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261)
Key features and details
- Rabbit polyclonal to Rab5 - Drosophila Early Endosome Marker
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
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Overview
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Product name
Anti-Rab5 antibody - Drosophila Early Endosome Marker
See all Rab5 primary antibodies -
Description
Rabbit polyclonal to Rab5 - Drosophila Early Endosome Marker -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Drosophila melanogaster -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Fruit fly (Drosophila melanogaster) Rab5.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
Target
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Function
Required for the fusion of plasma membranes and early endosomes. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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Alternative names
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
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Images
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Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261) at 1 µg/ml + Schneider L2 whole cell lysate (ab14893) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 135 kDa, 145 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab31261 detects a strong 26 kDa band in Drosophila lysate consistent with expected molecular weight of Rab5. In some batches of this antibody, we have observed additional bands at ~135, 145 and 70 kDa, the identity of which are unknown.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (62)
ab31261 has been referenced in 62 publications.
- O'Brien CE et al. The GARP complex prevents sterol accumulation at the trans-Golgi network during dendrite remodeling. J Cell Biol 222:N/A (2023). PubMed: 36239632
- Xu L et al. ER-mitochondrial contact protein Miga regulates autophagy through Atg14 and Uvrag. Cell Rep 41:111583 (2022). PubMed: 36323251
- Wagner K et al. Phospholipase D and retromer promote recycling of TRPL ion channel via the endoplasmic reticulum. Traffic 23:42-62 (2022). PubMed: 34719094
- Ding X et al. Toll-7 promotes tumour growth and invasion in Drosophila. Cell Prolif 55:e13188 (2022). PubMed: 35050535
- Tanasic D et al. Myosin V facilitates polarised E-cadherin secretion. Traffic 23:374-390 (2022). PubMed: 35575181