Key features and details
- Rabbit polyclonal to Rab5 - Drosophila Early Endosome Marker
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Product nameAnti-Rab5 antibody - Drosophila Early Endosome Marker
See all Rab5 primary antibodies
DescriptionRabbit polyclonal to Rab5 - Drosophila Early Endosome Marker
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Drosophila melanogaster
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab31261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
FunctionRequired for the fusion of plasma membranes and early endosomes.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationCell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.
- Information by UniProt
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261) at 1 µg/ml +
Schneider L2 whole cell lysate (ab14893) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 135 kDa, 145 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab31261 detects a strong 26 kDa band in Drosophila lysate consistent with expected molecular weight of Rab5. In some batches of this antibody, we have observed additional bands at ~135, 145 and 70 kDa, the identity of which are unknown.
ab31261 staining Rab5 in Drosophila S2 cells by Immunocytochemistry/ Immunofluorescence.
S2 cells were fixed in 4% paraformaldehyde for 10 minutes and then permeablized by incubating with 0.2% Triton X-100 in PBS for 5 minutes at room temperature. After blocking, cells were incubated overnight with ab31261 diluted in PBS containing 10% horse serum and 0.2% Triton X-100. A Fluorescein-conjugated anti-rabbit IgG was used as the secondary.
ab31261 has been referenced in 37 publications.
- Gualtieri M & Vaccari T Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster. Methods Mol Biol 1998:13-29 (2019). PubMed: 31250291
- Lorincz P et al. Vps8 overexpression inhibits HOPS-dependent trafficking routes by outcompeting Vps41/Lt. Elife 8:N/A (2019). PubMed: 31194677
- Negreiros E et al. N-linked glycosylation restricts the function of Short gastrulation to bind and shuttle BMPs. Development 145:N/A (2018). PubMed: 30355725
- Donohoe CD et al. Atf3 links loss of epithelial polarity to defects in cell differentiation and cytoarchitecture. PLoS Genet 14:e1007241 (2018). PubMed: 29494583
- Jin EJ et al. Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals. Curr Biol 28:1027-1038.e4 (2018). PubMed: 29551411