Product nameAnti-Rab5 antibody - Drosophila Early Endosome Marker
See all Rab5 primary antibodies
DescriptionRabbit polyclonal to Rab5 - Drosophila Early Endosome Marker
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Drosophila melanogaster
- This antibody gave a positive signal in Schneider L2 (Fruit fly (Drosophila melanogaster)) Whole Cell Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab31261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20404143|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).|
FunctionRequired for the fusion of plasma membranes and early endosomes.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationCell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.
- Information by UniProt
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
ab31261 staining 3rd instar wing imaginal disc cells of fruit fly (Drosophila melanogaster). Cells were formaldehyde fixed and blocked with 5 % serum for 2 hours at 22°C prior to incubating with the primary antibody, diluted 1/500, for 16 hours at 4°C. A Cy3® conjugated donkey anti-rabbit IgG (H+L) antibody, diluted 1/500, was used as the secondary.
In the image, the nuclei of some cells are marked by GFP (green). The punctate, non-nuclear staining pattern is indicative of endosomes (similar to other endosomal markers such as Hrs).
Anti-Rab5 antibody - Drosophila Early Endosome Marker (ab31261) at 1 µg/ml +
Schneider L2 whole cell lysate (ab14893) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Additional bands at: 135 kDa, 145 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
ab31261 detects a strong 26 kDa band in Drosophila lysate consistent with expected molecular weight of Rab5. In some batches of this antibody, we have observed additional bands at ~135, 145 and 70 kDa, the identity of which are unknown.
ab31261 staining Rab5 in Drosophila S2 cells by Immunocytochemistry/ Immunofluorescence.
S2 cells were fixed in 4% paraformaldehyde for 10 minutes and then permeablized by incubating with 0.2% Triton X-100 in PBS for 5 minutes at room temperature. After blocking, cells were incubated overnight with ab31261 diluted in PBS containing 10% horse serum and 0.2% Triton X-100. A Fluorescein-conjugated anti-rabbit IgG was used as the secondary.
This product has been referenced in:
- Donohoe CD et al. Atf3 links loss of epithelial polarity to defects in cell differentiation and cytoarchitecture. PLoS Genet 14:e1007241 (2018). Read more (PubMed: 29494583) »
- Jin EJ et al. Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals. Curr Biol 28:1027-1038.e4 (2018). Read more (PubMed: 29551411) »