Anti-Rab5 antibody - Early Endosome Marker (ab18211)
Key features and details
- Rabbit polyclonal to Rab5 - Early Endosome Marker
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Rab5 antibody - Early Endosome Marker
See all Rab5 primary antibodies -
Description
Rabbit polyclonal to Rab5 - Early Endosome Marker -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 43% homology with Rab5C. Cross-reactivity with this protein has not been confirmed experimentally. -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Dog, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Human Rab5 aa 150 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab18625) -
Positive control
- ICC: HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (15) |
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
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| ICC |
Use a concentration of 1 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
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ICC
Use a concentration of 1 µg/ml. |
Target
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Function
Required for the fusion of plasma membranes and early endosomes. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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Database links
- Entrez Gene: 404008 Dog
- Entrez Gene: 5868 Human
- Entrez Gene: 271457 Mouse
- Entrez Gene: 64633 Rat
- Omim: 179512 Human
- SwissProt: P18066 Dog
- SwissProt: P20339 Human
- SwissProt: Q9CQD1 Mouse
see all -
Alternative names
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
see all
Images
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Immunocytochemistry - Anti-Rab5 antibody - Early Endosome Marker (ab18211)
ab18211 staining Rab5 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab18211 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211)Anti-Rab5 antibody - Early Endosome Marker (ab18211) at 1 µg/ml + Jurkat cell lysate at 20 µg
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Additional bands at: 40 kDa, 70 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211)All lanes : Anti-Rab5 antibody - Early Endosome Marker (ab18211) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770)
Lane 3 : Brain (Mouse) Tissue Lysate (ab27253)
Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 5 : Spinal Cord (Mouse) Tissue Lysate
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 7 : Brain (Rat) Tissue Lysate - normal tissue
Lane 8 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Western blot - Anti-Rab5 antibody - Early Endosome Marker (ab18211)Anti-Rab5 antibody - Early Endosome Marker (ab18211) at 1 µg/ml +Recombinant Human Rab5A protein (ab62956) at 0.001 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Exposure time: 150 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (96)
ab18211 has been referenced in 96 publications.
- Gautier MK & Ginsberg SD A method for quantification of vesicular compartments within cells using 3D reconstructed confocal z-stacks: Comparison of ImageJ and Imaris to count early endosomes within basal forebrain cholinergic neurons. J Neurosci Methods 350:109038 (2021). PubMed: 33338543
- Lv J et al. Distinct uptake, amplification, and release of SARS-CoV-2 by M1 and M2 alveolar macrophages. Cell Discov 7:24 (2021). PubMed: 33850112
- Vandendriessche C et al. Importance of extracellular vesicle secretion at the blood-cerebrospinal fluid interface in the pathogenesis of Alzheimer's disease. Acta Neuropathol Commun 9:143 (2021). PubMed: 34425919
- Huang X et al. Nanotopography Enhances Dynamic Remodeling of Tight Junction Proteins through Cytosolic Liquid Complexes. ACS Nano 14:13192-13202 (2020). PubMed: 32940450
- Pensalfini A et al. Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease. Cell Rep 33:108420 (2020). PubMed: 33238112