Anti-Rab5 antibody - Early Endosome Marker (ab18211)
Key features and details
- Rabbit polyclonal to Rab5 - Early Endosome Marker
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Rab5 antibody - Early Endosome Marker
See all Rab5 primary antibodies -
Description
Rabbit polyclonal to Rab5 - Early Endosome Marker -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 43% homology with Rab5C. Cross-reactivity with this protein has not been confirmed experimentally.
Replenishment batches of our polyclonal antibody, ab18211 are tested in WB. Previous batches were additionally validated in ICC. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab218624.
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Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Dog, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Human Rab5 aa 150 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab18625) -
Positive control
- ICC: HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (15) |
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
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ICC |
Use a concentration of 1 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). |
ICC
Use a concentration of 1 µg/ml. |
Target
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Function
Required for the fusion of plasma membranes and early endosomes. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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Database links
- Entrez Gene: 404008 Dog
- Entrez Gene: 5868 Human
- Entrez Gene: 271457 Mouse
- Entrez Gene: 64633 Rat
- Omim: 179512 Human
- SwissProt: P18066 Dog
- SwissProt: P20339 Human
- SwissProt: Q9CQD1 Mouse
see all -
Alternative names
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
see all
Images
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ab18211 staining Rab5 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab18211 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Anti-Rab5 antibody - Early Endosome Marker (ab18211) at 1 µg/ml + Jurkat cell lysate at 20 µg
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDa
Additional bands at: 40 kDa, 70 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-Rab5 antibody - Early Endosome Marker (ab18211) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab46770)
Lane 3 : Brain (Mouse) Tissue Lysate (ab27253)
Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 5 : Spinal Cord (Mouse) Tissue Lysate
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 7 : Brain (Rat) Tissue Lysate - normal tissue
Lane 8 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Anti-Rab5 antibody - Early Endosome Marker (ab18211) at 1 µg/ml +
Recombinant Human Rab5A protein (ab62956) at 0.001 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Exposure time: 150 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (103)
ab18211 has been referenced in 103 publications.
- Mareninova OA et al. Rab9 Mediates Pancreatic Autophagy Switch From Canonical to Noncanonical, Aggravating Experimental Pancreatitis. Cell Mol Gastroenterol Hepatol 13:599-622 (2022). PubMed: 34610499
- Giridharan SSP et al. Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes. Elife 11:N/A (2022). PubMed: 35040777
- Ohka S et al. The uncoating of EV71 in mature late endosomes requires CD-M6PR. Biol Open 11:N/A (2022). PubMed: 35929543
- Park SS et al. Temsirolimus Enhances Anti-Cancer Immunity by Inducing Autophagy-Mediated Degradation of the Secretion of Small Extracellular Vesicle PD-L1. Cancers (Basel) 14:N/A (2022). PubMed: 36077620
- Jiang W et al. RagC GTPase regulates mTOR to promote chemoresistance in senescence-like HepG2 cells. Front Physiol 13:949737 (2022). PubMed: 36267578