Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Miapaca2)
Total protein in input
1000 µg
Treatment
EGF 50 ng/ml, for 16 h
Immuno-precipitation step
Protein A/G
Specification
Miapaca2
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
1 µg/mg lysate
Western Blot antibody
Dilution
1/200
Name
Non-Abcam antibody was used: RAB5 from c.s. + cleanblot
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
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Submitted Jun 16 2016