Recombinant Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5438] to Rab5 - Early Endosome Marker
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Rab5 antibody [EPR5438] - Early Endosome Marker
See all Rab5 primary antibodies -
Description
Rabbit monoclonal [EPR5438] to Rab5 - Early Endosome Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Rab5 aa 150-250. The exact sequence is proprietary.
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Positive control
- 293T, HeLa and Jurkat whole cell lysate (ab7899); Human brain tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5438 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 24 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
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Function
Required for the fusion of plasma membranes and early endosomes. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes. - Information by UniProt
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Database links
- Entrez Gene: 5868 Human
- Entrez Gene: 271457 Mouse
- Entrez Gene: 64633 Rat
- Omim: 179512 Human
- SwissProt: P20339 Human
- SwissProt: Q9CQD1 Mouse
- SwissProt: M0RC99 Rat
- Unigene: 475663 Human
see all -
Alternative names
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
see all
Images
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![Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)](https://www.abcam.com/ps/products/109/ab109534/Images/ab109534-276554-anti-rab5-antibody-epr5438-early-endosome-marker-western-blot.jpg)
Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Rab5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Hu brain whole cell lysate (20 µg)
Lane 4: Ms brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109534 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)](https://www.abcam.com/ps/products/109/ab109534/Images/ab109534-239659-anti-rab5-antibody-epr5438-early-endosome-marker-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)Immunohistochemical staining of paraffin embedded human pancreas with purified ab109534 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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![Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)](https://www.abcam.com/ps/products/109/ab109534/Images/ab109534-239658-anti-rab5-antibody-epr5438-early-endosome-marker-western-blot.jpg)
Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)All lanes : purified at 1/5000 dilution
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)](https://www.abcam.com/ps/products/109/ab109534/Images/ab109534-272270-anti-rab5-antibody-epr5438-early-endosome-marker-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)This image is courtesy of an anonymous abreview.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Pancreas tissue sections (with ADM and PanIN lesions) from KC mice labeling Rab5 with ab109534 at 1/200 dilution. Tissue was fixed with paraformaldehyde and permeabilized with TritonX100 0.1% for 10 min at room temperature. Heat-mediated antigen retrieval was performed using TRIS-EDTA pH9 buffer. Tissue sections were blocked with 10% goat serum for 1 hour at 20°C. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/500 dilution.
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![Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)](https://www.abcam.com/ps/products/109/ab109534/Images/ab109534-239655-anti-rab5-antibody-epr5438-early-endosome-marker-western-blot.jpg)
Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/5000 dilution (purified)
Lane 1 : MCF7 cell lysate
Lane 2 : HEK293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 24 kDa
Observed band size: 24 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)](https://www.abcam.com/ps/products/109/ab109534/Images/ab109534-122474-anti-rab5-antibody-epr5438-early-endosome-marker-western-blot.jpg)
Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 24 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)](https://www.abcam.com/ps/products/109/ab109534/Images/ab109534-122473-anti-rab5-antibody-epr5438-early-endosome-marker-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)Immunohistochemical staining of Rab5 in paraffin embedded human brain tissue with unpurified ab109534, at 1/100 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Protocols
References
ab109534 has been referenced in 7 publications.
- Sharoar MG et al. Sequential formation of different layers of dystrophic neurites in Alzheimer's brains. Mol Psychiatry N/A:N/A (2019). PubMed: 30899091
- Palamidessi A et al. Unjamming overcomes kinetic and proliferation arrest in terminally differentiated cells and promotes collective motility of carcinoma. Nat Mater 18:1252-1263 (2019). PubMed: 31332337
- Fermaintt CS et al. A bioactive mammalian disaccharide associated with autoimmunity activates STING-TBK1-dependent immune response. Nat Commun 10:2377 (2019). PubMed: 31147550
- Soung YH et al. The Role of Arrestin Domain-Containing 3 in Regulating Endocytic Recycling and Extracellular Vesicle Sorting of Integrin ß4 in Breast Cancer. Cancers (Basel) 10:N/A (2018). PubMed: 30545011
- Chen S et al. Identification of an Essential Region for Translocation of Clostridium difficile Toxin B. Toxins (Basel) 8:N/A (2016). PubMed: 27537911
- Schindler C et al. EARP is a multisubunit tethering complex involved in endocytic recycling. Nat Cell Biol 17:639-50 (2015). PubMed: 25799061
- Zarovni N et al. Integrated isolation and quantitative analysis of exosome shuttled proteins and nucleic acids using immunocapture approaches. Methods 87:46-58 (2015). PubMed: 26044649
