Name: Anti-Rab5 antibody [EPR5438] - Early Endosome Marker
Brand: RabMAb
SKU: ab109534
Rating: 4 (8 reviews)

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Product name

Anti-Rab5 antibody [EPR5438] - Early Endosome Marker
See all Rab5 primary antibodies
Description

Rabbit monoclonal [EPR5438] to Rab5 - Early Endosome Marker
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human Rab5 aa 150-250. The exact sequence is proprietary.
Positive control
- 293T, HeLa and Jurkat whole cell lysate (ab7899); Human brain tissue.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5438
Isotype

IgG
Research areas

Alternative Versions
- Anti-Rab5 antibody [EPR5438] - BSA and Azide free (ab234908)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa membrane extract lysate (ab29547)
- Jurkat membrane extract lysate (ab30130)
Recombinant Protein
- Recombinant Human Rab5 protein (ab62956)

Our Abpromise guarantee covers the use of ab109534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt,ICC/IF or IP.

Function

Required for the fusion of plasma membranes and early endosomes.
Sequence similarities

Belongs to the small GTPase superfamily. Rab family.
Cellular localization

Cell membrane. Early endosome membrane. Melanosome. Enriched in stage I melanosomes.
Target information above from: UniProt accession P20339 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5868 Human
- Entrez Gene: 271457 Mouse
- Entrez Gene: 64633 Rat
- Omim: 179512 Human
- SwissProt: P20339 Human
- SwissProt: Q9CQD1 Mouse
- SwissProt: M0RC99 Rat
- Unigene: 475663 Human
- Unigene: 329123 Mouse
- Unigene: 44477 Rat
see all
Alternative names
- RAB 5 antibody
- RAB 5A antibody
- RAB5A antibody
- RAB5A member RAS oncogene family antibody
- RAB5A_HUMAN antibody
- RAS associated protein RAB5A antibody
- Ras related protein Rab 5A antibody
- Ras-related protein Rab-5A antibody
see all

Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Rab5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Hu brain whole cell lysate (20 µg)
Lane 4: Ms brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109534 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

Immunohistochemical staining of paraffin embedded human pancreas with purified ab109534 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

All lanes : purified at 1/5000 dilution

Lane 1 : Mouse brain
Lane 2 : Rat brain

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 24 kDa
Observed band size: 24 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)This image is courtesy of an anonymous abreview.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Pancreas tissue sections (with ADM and PanIN lesions) from KC mice labeling Rab5 with ab109534 at 1/200 dilution. Tissue was fixed with paraformaldehyde and permeabilized with TritonX100 0.1% for 10 min at room temperature. Heat-mediated antigen retrieval was performed using TRIS-EDTA pH9 buffer. Tissue sections were blocked with 10% goat serum for 1 hour at 20°C. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/500 dilution.
See Abreview
Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/5000 dilution (purified)

Lane 1 : MCF7 cell lysate
Lane 2 : HEK293 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 24 kDa
Observed band size: 24 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/1000 dilution (unpurified)

Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 24 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

Immunohistochemical staining of Rab5 in paraffin embedded human brain tissue with unpurified ab109534, at 1/100 dilution.

Western blot protocols
Immunohistochemistry protocols

This product has been referenced in:

Sharoar MG et al. Sequential formation of different layers of dystrophic neurites in Alzheimer's brains. Mol Psychiatry N/A:N/A (2019). Read more (PubMed: 30899091) »
Soung YH et al. The Role of Arrestin Domain-Containing 3 in Regulating Endocytic Recycling and Extracellular Vesicle Sorting of Integrin ß4 in Breast Cancer. Cancers (Basel) 10:N/A (2018). Read more (PubMed: 30545011) »

See all 5 Publications for this product

Publishing research using ab109534? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 10 Abreviews or Q&A

Application

Western blot

Sample

African green monkey Cell lysate - whole cell (Kidney (COS7 cells))

Gel Running Conditions

Reduced Denaturing (4-12% gel)

Loading amount

30 µg

Specification

Kidney (COS7 cells)

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 29 2017

Application

Western blot

Sample

Mouse Tissue lysate - whole (Brain)

Gel Running Conditions

Reduced Denaturing (4-12% gel)

Loading amount

30 µg

Specification

Brain

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 29 2017

Application

Western blot

Sample

Human Cell lysate - whole cell (Kidney (HEK293 cells))

Gel Running Conditions

Reduced Denaturing (4-12% gel)

Loading amount

30 µg

Specification

Kidney (HEK293 cells)

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 29 2017

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Murine Pancreatic tissue)

Permeabilization

Yes - Triton X100 0.01%

Specification

Murine Pancreatic tissue

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 25 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Pancreas (with ADM and PanIN lesions) from KC mice)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TRIS-EDTA pH9

Permeabilization

Yes - TritonX100 0.1% 10 min RT

Specification

Pancreas (with ADM and PanIN lesions) from KC mice

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 06 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (Pancreatic Cancer)

Gel Running Conditions

Reduced Denaturing (12.5%)

Loading amount

30 µg

Specification

Pancreatic Cancer

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 01 2016

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HepaRG)

Specification

HepaRG

Fixative

Formaldehyde

Permeabilization

Yes - 0.1% TritonX-100

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Mrs. Alina Macovei

Verified customer

Submitted May 16 2013

Application

Western blot

Sample

Human Cell lysate - whole cell (HepaRG)

Loading amount

20 µg

Specification

HepaRG

Gel Running Conditions

Reduced Denaturing (10%)

Blocking step

Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Mrs. Alina Macovei

Verified customer

Submitted May 15 2013

Question

I would like to know what is the concentration of this antibody:
Rab5a 3768-1 Epitomics lot YH112425.

Abcam community

Verified customer

Asked on Apr 03 2013

Answer

The IgG concentration of Abcam ab109534 lot GR68319 (which is the same as Rab5a 3768-1 Epitomics lot YH112425) is 0.032 mg/ml. This is lower than the IgG concentration of most mouse monoclonal and rabbit polyclonal antibodies but is not unusual for rabbit monoclonals, which generally have much higher affinity for their targets than either mouse monoclonals or rabbit polyclonals.

Abcam Scientific Support

Answered on Apr 03 2013

Question

Would it be possible to get the concentrations of the current lots of the following Abs?

3768-1

3660-1

Abcam community

Verified customer

Asked on Feb 01 2013

Answer

The IgG concentrations of the current lots are:

3768-1 (ab109534, lot GR68319) - 0.032 mg/ml

3660-1 (ab125068, lot GR75050) - 1.554 mg/ml

Abcam Scientific Support

Answered on Feb 01 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Western blot abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

Western blot abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

Western blot abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

Western blot abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab5 antibody [EPR5438]

Western blot abreview for Anti-Rab5 antibody [EPR5438]