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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

Reviews (9)Q&A (2)References (11)
Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
  • Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
  • Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
  • Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
  • Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5438] to Rab5 - Early Endosome Marker
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Rab5 antibody [EPR5438] - Early Endosome Marker
    See all Rab5 primary antibodies

  • Description

    Rabbit monoclonal [EPR5438] to Rab5 - Early Endosome Marker

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, HeLa and Jurkat whole cell lysate (ab7899); Human brain tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (5)
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IHC-P (1)
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

Images

  • Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
    Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Rab5 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: Hu brain whole cell lysate (20 µg)
    Lane 4: Ms brain whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109534 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

    Immunohistochemical staining of paraffin embedded human pancreas with purified ab109534 at a working dilution of 1 in 1000. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
    Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
    All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/5000 dilution (purified)

    Lane 1 : Mouse brain
    Lane 2 : Rat brain

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)This image is courtesy of an anonymous abreview.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Pancreas tissue sections (with ADM and PanIN lesions) from KC mice labeling Rab5 with ab109534 at 1/200 dilution. Tissue was fixed with paraformaldehyde and permeabilized with TritonX100 0.1% for 10 min at room temperature. Heat-mediated antigen retrieval was performed using TRIS-EDTA pH9 buffer. Tissue sections were blocked with 10% goat serum for 1 hour at 20°C. A polyclonal goat anti-rabbit Alexa Fluor 488 secondary antibody was used at 1/500 dilution. 

    See Abreview

  • Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
    Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
    All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/5000 dilution (purified)

    Lane 1 : MCF7 cell lysate
    Lane 2 : HEK293 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
    Western blot - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
    All lanes : Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534) at 1/1000 dilution (unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

    Immunohistochemical staining of Rab5 in paraffin embedded human brain tissue with unpurified ab109534, at 1/100 dilution.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)
    Anti-Rab5 antibody [EPR5438] - Early Endosome Marker (ab109534)

References (11)

Publishing research using ab109534? Please let us know so that we can cite the reference in this datasheet.

ab109534 has been referenced in 11 publications.

  • Sharoar MG  et al. Accumulation of saposin in dystrophic neurites is linked to impaired lysosomal functions in Alzheimer's disease brains. Mol Neurodegener 16:45 (2021). PubMed: 34215298
  • Rocha EM  et al. LRRK2 inhibition prevents endolysosomal deficits seen in human Parkinson's disease. Neurobiol Dis 134:104626 (2020). PubMed: 31618685
  • Pensalfini A  et al. Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease. Cell Rep 33:108420 (2020). PubMed: 33238112
  • Underwood R  et al. The GTPase Rab27b regulates the release, autophagic clearance, and toxicity of a-synuclein. J Biol Chem 295:8005-8016 (2020). PubMed: 32350025
  • Sharoar MG  et al. Sequential formation of different layers of dystrophic neurites in Alzheimer's brains. Mol Psychiatry N/A:N/A (2019). PubMed: 30899091
View all Publications for this product

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Rab5 antibody [EPR5438] - Early Endosome Marker

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas (with ADM and PanIN lesions) from KC mice)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA pH9
Permeabilization
Yes - TritonX100 0.1% 10 min RT
Specification
Pancreas (with ADM and PanIN lesions) from KC mice
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
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Verified customer

Submitted Dec 06 2016

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