Overview

  • Product name

    Anti-RAB5C/RABL antibody [EPR17321]
    See all RAB5C/RABL primary antibodies
  • Description

    Rabbit monoclonal [EPR17321] to RAB5C/RABL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human RAB5C/RABL aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P51148

  • Positive control

    • WB: HEK293, HepG2 and U937 cell lysates; Human fetal heart lysate, IHC-P: Human kidney tissue. ICC/IF: HepG2 and MCF7 cells,
  • General notes

     This product was previously labelled as RAB5C

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.

Target

  • Function

    Protein transport. Probably involved in vesicular traffic.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Cell membrane. Early endosome membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • L1880 antibody
    • RAB, member of RAS oncogene family like antibody
    • RAB5C antibody
    • RAB5C, member of RAS oncogene family antibody
    • RAB5C, member RAS oncogene family antibody
    • RAB5C_HUMAN antibody
    • RAB5CL antibody
    • RAB5L antibody
    • RABL antibody
    • Ras-related protein Rab-5C antibody
    see all

Images

  • All lanes : Anti-RAB5C/RABL antibody [EPR17321] (ab199530) at 1/1000 dilution

    Lane 1 : HEK293 (Human embryonic kidney) cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Ab199530 staining RAB5C/RABL in HepG2 cells (Human hepatocellular carcinoma epithelial cell) by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 100% Methanol. Samples were incubated with primary antibody at 1:500 dilution (2.2µg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), ab195889 was used as a counterstain antibody at 1:200 dilution (2.5µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining in HepG2 cells.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RAB5C/RABL with ab199530 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining of Human kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody and secondary antibody Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Anti-RAB5C/RABL antibody [EPR17321] (ab199530) at 1/10000 dilution + U937 (Human histiocytic lymphoma cells) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-RAB5C/RABL antibody [EPR17321] (ab199530) at 1/1000 dilution + Human fetal heart tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab199530 has not yet been referenced specifically in any publications.

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