Product nameAnti-RAB5C/RABL antibody [EPR17321] (HRP)
See all RAB5C/RABL primary antibodies
DescriptionRabbit monoclonal [EPR17321] to RAB5C/RABL (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human RAB5C/RABL aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P51148
- WB: HEK293, HepG2 cell lysates, Human Fetal Heart tissue lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab209475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
FunctionProtein transport. Probably involved in vesicular traffic.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationCell membrane. Early endosome membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- L1880 antibody
- RAB, member of RAS oncogene family like antibody
- RAB5C antibody
All lanes : Anti-RAB5C/RABL antibody [EPR17321] (HRP) (ab209475) at 1/5000 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Brain (Human) Tissue Lysate - fetal normal tissue
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 30 seconds
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209475 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab209475 has not yet been referenced specifically in any publications.