Overview

  • Product name

    Anti-RAB5C/RABL antibody [EPR17321] (HRP)
    See all RAB5C/RABL primary antibodies
  • Description

    Rabbit monoclonal [EPR17321] to RAB5C/RABL (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human RAB5C/RABL aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P51148

  • Positive control

    • WB: HEK293, HepG2 cell lysates, Human Fetal Heart tissue lysate
  • General notes

     

     

     This product was previously labelled as RAB5C

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

Target

  • Function

    Protein transport. Probably involved in vesicular traffic.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Cell membrane. Early endosome membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • L1880 antibody
    • RAB, member of RAS oncogene family like antibody
    • RAB5C antibody
    • RAB5C, member of RAS oncogene family antibody
    • RAB5C, member RAS oncogene family antibody
    • RAB5C_HUMAN antibody
    • RAB5CL antibody
    • RAB5L antibody
    • RABL antibody
    • Ras-related protein Rab-5C antibody
    see all

Images

  • All lanes : Anti-RAB5C/RABL antibody [EPR17321] (HRP) (ab209475) at 1/5000 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Brain (Human) Tissue Lysate - fetal normal tissue

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209475 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab209475 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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