• Product name

  • Description

    Rabbit polyclonal to RAB7
  • Host species

  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Chicken, Cow, Dog, Human, Xenopus laevis, Chimpanzee, Monkey, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a portion of the amino acids 90-140 of Human RAB7A

  • Positive control

    • Skeletal muscle tissue.



Our Abpromise guarantee covers the use of ab77993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22781172
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P Use a concentration of 10 µg/ml.


  • Function

    Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation.
  • Tissue specificity

    Widely expressed; high expression found in skeletal muscle.
  • Involvement in disease

    Defects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B) [MIM:600882]; also known as hereditary motor and sensory neuropathy II (HMSN2). CMT2B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2B is clinically characterized by marked distal muscle weakness and a high frequency of foot ulcers, infections and amputations of the toes. CMT2B inheritance is autosomal dominant.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Late endosome. Lysosome. Cytoplasmic vesicle > phagosome. Melanosome. Cytoplasmic vesicle > phagosome membrane. Co-localizes with OSBPL1A at the late endosome. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMT2B antibody
    • PRO2706 antibody
    • PSN antibody
    • RAB7, member RAS oncogene family antibody
    • RAB7A antibody
    • RAB7A, member RAS oncogene family antibody
    • RAB7A_HUMAN antibody
    • Ras associated protein RAB7 antibody
    • Ras related protein Rab7 antibody
    • Ras related protein Rab7a antibody
    • Ras-related protein Rab-7a antibody
    see all


  • ab77993 at 10µg/ml staining RAB7 in skeletal muscle tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • All lanes : Anti-RAB7 antibody (ab77993) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa
    Additional bands at: 34 kDa, 49 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 1 minute


This product has been referenced in:

  • Villella VR  et al. A pathogenic role for cystic fibrosis transmembrane conductance regulator in celiac disease. EMBO J 38:N/A (2019). Read more (PubMed: 30498130) »
  • Cioni JM  et al. Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons. Cell 176:56-72.e15 (2019). Read more (PubMed: 30612743) »
See all 11 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


Comme convenu j'ai mis en place l'envoi d'une unité de ab77993, le numéro de commande de remplacement gratuit de ce produit est ****.
Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.
Veuillez noter que cet anticorps de remplacement est également couvert par la garantie Abpromise, donc n'hésitez pas à nous contacter de nouveau si nécessaire.

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Thank you for contacting us. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.
Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.
I apologize for the inconvenience and am pleased to offer you a free of charge replacement with a different lot, credit note, or refund in compensation.
Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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I used the anti-Rab7 antibody to stain Vero and HeLa cells and did not observe any staining at all. Cells were seeded at 150,000 cells per well onto glass coverslips in a 6 well tissue culture plate and allowed to adhere overnight. Cells were washed with PBS 3x and fixed with 4% PFA for 10 min at room temp, and permeabilized with 0.5 % Triton X for 1 minute. Cells were washed with PBS 3x by 5 min each. Cells were blocked with 5% goat serum, 5% BSA prepared in PBS-Tween 0.5% for 1 hour at room temp, and incubated with primary rabbit-antiRab7 antibody (1:50 diluted in block) either for 1 hr at 37C, overnight at room temp, or overnight at 4C. Next day, cells were washed with PBS-Tween 0.5% and stained with secondary antibody (Fab fragment goat anti-H+L IgG Rabbit DyLight 649 at 1:200 dilution in block (1 hr at 37C) (Jackson immuno Research cat:111-497-003). Samples were washed 3x by 5 min with PBS and mounted with ProLong Gold containing DAPI and imaged using Leica TCS SP5 Confocal and multiphoton microscope. In addition to the 3 different primary antibody incubation conditions with 4% PFA, I also tried to fix cells with 1% glutaraldehyde with a sodium borohydride reduction and also did not observe any staining. I noticed that another customer also had a problem staining Vero cells with this antibody so I inquired about this with customer support and was assured that the problem was likely with the lot of antibody and that I should still go ahead with purchasing this antibody. However, it seems that it did not work for me either in both the human and monkey cells.

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Thank you for contacting us. I am sorry to hear that ab77993 is giving no signal on your samples. I would expect the protocol you are using to work well. If you have purchased the antibody in the past six months, I would be happy to offer you a free of charge replacement with one of our other RAB7 antibodies (such as ab50533) or a credit or refund. Please let me know your original order or PO number and how you would like to proceed and I will be happy to help you further.

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab50533.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Immunocytochemistry/ Immunofluorescence
Chicken Cell (chicken embryonic epithelial cell)
chicken embryonic epithelial cell
Yes - 0.1% Tween
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C

Dr. Lisa Taneyhill

Verified customer

Submitted Feb 22 2010

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