Key features and details
- Rabbit polyclonal to RAB7
- Suitable for: IHC-Fr, WB, IHC-P
- Reacts with: Rat, Chicken, Cow, Dog, Human, Xenopus laevis, Chimpanzee, Monkey, Zebrafish
- Isotype: IgG
Product nameAnti-RAB7 antibody
See all RAB7 primary antibodies
DescriptionRabbit polyclonal to RAB7
Tested applicationsSuitable for: IHC-Fr, WB, IHC-Pmore details
Species reactivityReacts with: Rat, Chicken, Cow, Dog, Human, Xenopus laevis, Chimpanzee, Monkey, Zebrafish
Synthetic peptide, corresponding to a portion of the amino acids 90-140 of Human RAB7A
- Skeletal muscle tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab77993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22781172|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionKey regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation.
Tissue specificityWidely expressed; high expression found in skeletal muscle.
Involvement in diseaseDefects in RAB7A are the cause of Charcot-Marie-Tooth disease type 2B (CMT2B) [MIM:600882]; also known as hereditary motor and sensory neuropathy II (HMSN2). CMT2B is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2B is clinically characterized by marked distal muscle weakness and a high frequency of foot ulcers, infections and amputations of the toes. CMT2B inheritance is autosomal dominant.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationLate endosome. Lysosome. Cytoplasmic vesicle > phagosome. Melanosome. Cytoplasmic vesicle > phagosome membrane. Co-localizes with OSBPL1A at the late endosome. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium.
- Information by UniProt
- CMT2B antibody
- PRO2706 antibody
- PSN antibody
ab77993 at 10µg/ml staining RAB7 in human skeletal muscle tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
All lanes : Anti-RAB7 antibody (ab77993) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 34 kDa, 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
ab77993 has been referenced in 12 publications.
- Villella VR et al. A pathogenic role for cystic fibrosis transmembrane conductance regulator in celiac disease. EMBO J 38:N/A (2019). PubMed: 30498130
- Cioni JM et al. Late Endosomes Act as mRNA Translation Platforms and Sustain Mitochondria in Axons. Cell 176:56-72.e15 (2019). PubMed: 30612743
- Qin Y et al. CID1067700, a late endosome GTPase Rab7 receptor antagonist, attenuates brain atrophy, improves neurologic deficits and inhibits reactive astrogliosis in rat ischemic stroke. Acta Pharmacol Sin 40:724-736 (2019). PubMed: 30315251
- Saitoh S et al. Rab5-regulated endocytosis plays a crucial role in apical extrusion of transformed cells. Proc Natl Acad Sci U S A 114:E2327-E2336 (2017). PubMed: 28270608
- Toschi P et al. Evidence of Placental Autophagy during Early Pregnancy after Transfer of In Vitro Produced (IVP) Sheep Embryos. PLoS One 11:e0157594 (2016). WB . PubMed: 27326761