Western blot abreview for Anti-RAB7 antibody [EPR7589]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293RT, HCT116)
Gel Running Conditions
Reduced Denaturing
Loading amount
35 µg
Specification
HEK293RT, HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibo
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
odyssey clx
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 20 kDa

Additional data

Additional Notes
lane1: HEK293T, lane2: HCT116

Abcam user community

Verified customer

Submitted Aug 31 2019

Sign up