Overview

  • Product name

    Anti-RAB7B antibody [EPR15727]
    See all RAB7B primary antibodies
  • Description

    Rabbit monoclonal [EPR15727] to RAB7B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RAB7B aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96AH8

  • Positive control

    • THP-1, 293, U937 and NIH/3T3 cell lysates; Human skeletal muscle and prostatic hyperplasia tissues; U937 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab193360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/270.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/2000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/150.

Target

  • Function

    Controls vesicular trafficking from endosomes to the trans-Golgi network (TGN). Acts as a negative regulator of TLR9 signaling and can suppress TLR9-triggered TNFA, IL6, and IFNB production in macrophages by promoting TLR9 lysosomal degradation. Also negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Promotes megakaryocytic differentiation by increasing NF-kappa-B-dependent IL6 production and subsequently enhancing the association of STAT3 with GATA1. Not involved in the regulation of the EGF- and EGFR degradation pathway.
  • Tissue specificity

    Expressed in heart, placenta, lung, skeletal muscle and peripheral blood leukocyte.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Late endosome. Lysosome. Golgi apparatus > trans-Golgi network. Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC16212 antibody
    • MGC9726 antibody
    • RAB7 antibody
    • rab7B antibody
    • RAB7B, member RAS oncogene family antibody
    • RAB7B_HUMAN antibody
    • Ras related protein Rab 7b antibody
    • Ras-related protein Rab-7B antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling RAB7B with ab193360 at 1/150 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

  • All lanes : Anti-RAB7B antibody [EPR15727] (ab193360) at 1/1000 dilution

    Lane 1 : THP-1 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : U937 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-RAB7B antibody [EPR15727] (ab193360) at 1/1000 dilution + NIH/3T3 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling RAB7B with ab193360 at 1/150 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed U937 cells labeling RAB7B with ab193360 at 1/270 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

ab193360 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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