Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14873] to RAB8A - C-terminal (HRP)
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Rat, Human
- Conjugation: HRP
Product nameAnti-RAB8A antibody [EPR14873] - C-terminal (HRP)
See all RAB8A primary antibodies
DescriptionRabbit monoclonal [EPR14873] to RAB8A - C-terminal (HRP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RAB8A aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: P61006
- WB: HeLa, HCT 116, PC12 and wild-type HAP1 whole cell lysates. IHC-P: Normal human colon tissue sections.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
- Anti-RAB8A antibody [EPR14873] (ab188574)
- Anti-RAB8A antibody [EPR14873] - C-terminal (Alexa Fluor® 647) (ab207548)
- Anti-RAB8A antibody [EPR14873] - C-terminal (PE) (ab210666)
- Anti-RAB8A antibody [EPR14873] - C-terminal (Alexa Fluor® 555) (ab212004)
- Anti-RAB8A antibody [EPR14873] - C-terminal (Alexa Fluor® 568) (ab212005)
- Anti-RAB8A antibody [EPR14873] - C-terminal (Alexa Fluor® 594) (ab212006)
- Anti-RAB8A antibody [EPR14873] - Low endotoxin, Azide free (ab221780)
Our Abpromise guarantee covers the use of ab209046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).|
FunctionMay be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationCell membrane. Golgi apparatus. Cytoplasm > perinuclear region. Cell projection. Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis.
- Information by UniProt
- AA409338 antibody
- MEL antibody
- Mel transforming oncogene (derived from cell line NK14) RAB8 homolog antibody
IHC image of RAB8A staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab209046, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : Anti-RAB8A antibody [EPR14873] - C-terminal (HRP) (ab209046) at 1/5000 dilution
Lane 1 :
HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate at 10 µg
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 4 : Wild-type HAP1 cell lysate at 20 µg
Lane 5 : RAB8A knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209046 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab209046 has not yet been referenced specifically in any publications.