Overview

  • Product name

    Anti-RAB8A antibody [MJF-R22]
    See all RAB8A primary antibodies
  • Description

    Rabbit monoclonal [MJF-R22] to RAB8A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human RAB8A aa 1 to the C-terminus. Antibody epitope is C-terminal amino acids KITPDQQ (AA190-196)
    Database link: P61006

  • Positive control

    • WB: A549, MCF7, NIH/3T3, PC-12 and C6 whole cell lysates. IP: A549, NIH/3T3 and MEF whole cell lysates.
  • General notes

    This antibody was developed with support from The Michael J. Fox Foundation.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    MJF-R22
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IP 1/30.

Target

  • Function

    May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Cell membrane. Golgi apparatus. Cytoplasm > perinuclear region. Cell projection. Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA409338 antibody
    • MEL antibody
    • Mel transforming oncogene (derived from cell line NK14) RAB8 homolog antibody
    • Mel transforming oncogene (derived from cell line NK14) antibody
    • Mel transforming oncogene (RAB8 homolog) antibody
    • Mel transforming oncogene antibody
    • MGC124948 antibody
    • Oncogene c mel antibody
    • Oncogene c-mel antibody
    • RAB8 antibody
    • RAB8A antibody
    • RAB8A member RAS oncogene family antibody
    • RAB8A_HUMAN antibody
    • Ras associated protein RAB8 antibody
    • Ras related protein Rab 8A antibody
    • Ras-related protein Rab-8A antibody
    see all

Images

  • RAB8A was immunoprecipitated from varying amounts of MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate with 1 μg of ab237702 per IP test. Western blot was perfromed from the immunoprecipitate using ab237702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/2000 dilution.

    Lane 1: MEF whole cell lysate (input).
    Lane 2-7: ab237702 IP in different amounts of MEF lysates from 25 µg to 800 µg whole cell lysate.
    Lane 8: Pre-immune IgG instead of ab237702 in MEF whole cell lysate.

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting Buffer and concentration: 5% BSA/TBST.

    Exposure time: 10 seconds.

    This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.

  • RAB8A was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate using ab237702 at 1/30. Western blot was performed from the immunoprecipitate using ab237702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10μg (Input).
    Lane 2: ab237702 IP in NIH/3T3 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237702 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 seconds.

     

  • RAB8A was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell line) whole cell lysate using ab237702 at 1/30. Western blot was performed from the immunoprecipitate using ab237702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysate 10μg (Input).
    Lane 2: ab237702 IP in A459 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237702 in A549 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

     

  • All lanes : Anti-RAB8A antibody [MJF-R22] (ab237702) at 1/1000 dilution

    Lane 1 : Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 2 : Rab8A knockout A549 whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 5 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 6 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa


    Exposure time: 10 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

    The band around 50 kDa is non-specific band.

    The WT and Rab8A KO A549 lysates were kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.

References

ab237702 has not yet been referenced specifically in any publications.

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