Product nameAnti-RAB8A (phospho S111) antibody [MJF-R27-30]
See all RAB8A primary antibodies
DescriptionRabbit monoclonal [MJF-R27-30] to RAB8A (phospho S111)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human RAB8A aa 100-200 (phospho S111). The exact sequence is proprietary.
Database link: P61006
- WB: Wild-type HeLa (treated with 10 uM CCCP), whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab267492 in the following tested applications.
|WB||1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 24 kDa).|
FunctionMay be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.
Sequence similaritiesBelongs to the small GTPase superfamily. Rab family.
Cellular localizationCell membrane. Golgi apparatus. Cytoplasm > perinuclear region. Cell projection. Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis.
- Information by UniProt
- AA409338 antibody
- MEL antibody
- Mel transforming oncogene (derived from cell line NK14) RAB8 homolog antibody
All lanes : Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (ab267492) at 1/1000 dilution
Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) treated with DMSO
Lane 2 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) treated with 10 uM CCCP
Lane 3 : RAB8A knockout HeLa (human cervix adenocarcinoma epithelial cell) treated with DMSO
Lane 4 : RAB8A knockout HeLa (human cervix adenocarcinoma epithelial cell) treated with 10 uM CCCP
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 24 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
CCCP (carbonyl cyanide m-chlorophenyl hydrazone) inhibits oxidative phosphorylation.
The lysates were kindly provided by Pawan Singh Ph.D, University of Dundee.
Exposure time: 3min
ab267492 has not yet been referenced specifically in any publications.