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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)

Reviews (4) Submit a question References (10)
Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
  • Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
  • Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
  • Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
  • Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
  • Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
  • Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
  • Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13272] to Rab9 - Late Endosome Marker
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Rab9 antibody [EPR13272] - Late Endosome Marker
    See all Rab9 primary antibodies

  • Description

    Rabbit monoclonal [EPR13272] to Rab9 - Late Endosome Marker

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Rab9 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P51151

  • Positive control

    • WB: MDA-MB-231, MCF7, K562, HeLa, 293T and HepG2 whole cell lysate (ab7900). ICC/IF: HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
ICC/IF (1)
1/250.

For unpurified, use 1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
ICC/IF
1/250.

For unpurified, use 1/50 - 1/100.
Application notes
Is unsuitable for IHC-P.

Target

Images

  • Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
    Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
    All lanes : Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : RAB9A knockout HeLa cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : MDA-MB-231 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 23 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab179815 observed at 25 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab179815 Anti-Rab9 antibody [EPR13272]  was shown to specifically react with Rab9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265693 (knockout cell lysate ab257625) was used.  Wild-type and Rab9 knockout samples were subjected to SDS-PAGE.  ab179815 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)

    Immunofluorescence staining of HepG2 cells with purified ab179815 at a working dilution of 1/250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab179815 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
    Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
    Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815) at 1/2000 dilution (purified) + rat kidney at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
    Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
    Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815) at 1/2000 dilution (purified) + mouse spleen at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
    Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
    All lanes : Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815) at 1/2000 dilution (purified)

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
    Western blot - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
    All lanes : Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815) at 1/1000 dilution (unpurified)

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 23 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)

    Immunofluorescent staining of HepG2 cells labeling Rab9 with unpurified ab179815 at 1/50 dilution (red). DAPI nuclear staining (blue).

  • Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)
    Anti-Rab9 antibody [EPR13272] - Late Endosome Marker (ab179815)

References (10)

Publishing research using ab179815? Please let us know so that we can cite the reference in this datasheet.

ab179815 has been referenced in 10 publications.

  • Hattori K  et al. The Infantile Leukoencephalopathy-Associated Mutation of C11ORF73/HIKESHI Proteins Generates de novo Interactive Activity with Filamin A, Inhibiting Oligodendroglial Cell Morphological Differentiation. Medicines (Basel) 8:N/A (2021). PubMed: 33535532
  • Sawaguchi S  et al. Hypomyelinating Leukodystrophy 15 (HLD15)-Associated Mutation of EPRS1 Leads to Its Polymeric Aggregation in Rab7-Positive Vesicle Structures, Inhibiting Oligodendroglial Cell Morphological Differentiation. Polymers (Basel) 13:N/A (2021). PubMed: 33805425
  • Liu Y  et al. The overexpression of Rab9 promotes tumor progression regulated by XBP1 in breast cancer. Onco Targets Ther 12:1815-1824 (2019). PubMed: 30881034
  • Wang S  et al. Rational vaccinology with spherical nucleic acids. Proc Natl Acad Sci U S A 116:10473-10481 (2019). PubMed: 31068463
  • Xin X  et al. Drug-delivering-drug platform-mediated potent protein therapeutics via a non-endo-lysosomal route. Theranostics 8:3474-3489 (2018). PubMed: 30026860
  • Uchiyama K  et al. Prions amplify through degradation of the VPS10P sorting receptor sortilin. PLoS Pathog 13:e1006470 (2017). PubMed: 28665987
  • Kim SW  et al. Covalent, Non-Covalent, Encapsulated Nanodrug Regulate the Fate of Intra- and Extracellular Trafficking: Impact on Cancer and Normal Cells. Sci Rep 7:6454 (2017). PubMed: 28743942
  • Li RS  et al. Chiral nanoprobes for targeting and long-term imaging of the Golgi apparatus. Chem Sci 8:6829-6835 (2017). PubMed: 29147508
  • Ramaker JM  et al. Amyloid Precursor Proteins Are Dynamically Trafficked and Processed during Neuronal Development. Front Mol Neurosci 9:130 (2016). ICC/IF . PubMed: 27932950
  • Zhang C  et al. Biodegradable DNA-Brush Block Copolymer Spherical Nucleic Acids Enable Transfection Agent-Free Intracellular Gene Regulation. Small 11:5360-8 (2015). PubMed: 26297167

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Rab9 antibody [EPR13272] - Late Endosome Marker

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (bEnd.3)
Permeabilization
Yes - 0.1% Triton in PBS
Specification
bEnd.3
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Jul 03 2015

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