Overview

  • Product name

    Anti-Rab9 antibody [Mab9] - BSA and Azide free
    See all Rab9 primary antibodies
  • Description

    Mouse monoclonal [Mab9] to Rab9 - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    Recognizes prenylated and non-prenylated rab 9. This antibody does not cross-react with other rab family members.

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Dog Rab9.

  • Positive control

    • WB: HeLa; HEK293; Jurkat; HepG2; K562; IHC-P: human spleen and tonsil tissues, mouse testis tissue; FC:THP1 cells.
  • General notes

    Ab237960 is a PBS-only buffer format of ab2810. Please refer to ab2810 for recommended dilutions, protocols, and image data.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab237960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 10 µg/ml. Predicted molecular weight: 23 kDa.

Target

  • Function

    Involved in the transport of proteins between the endosomes and the trans Golgi network.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rab family.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2410064E05Rik antibody
    • AI195561 antibody
    • DmRab9 antibody
    • Rab 9 antibody
    • RAB 9A antibody
    • RAB9 member RAS oncogene family antibody
    • RAB9A antibody
    • RAB9A member RAS oncogene family antibody
    • RAB9A_HUMAN antibody
    • RAS ASSOCIATED PROTEIN RAB9 antibody
    • Ras related protein Rab 9A antibody
    • Ras-related protein Rab-9A antibody
    • Sid6061p antibody
    • Sid99 antibody
    see all

Images

  • ab2810 (2µg/ml) staining Rab9 in human spleen, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and cell membrane staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (ab2810).

  • Overlay histogram showing THP1 cells stained with ab2810 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2810, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (ab2810).

  • IHC image of Rab9 staining in human normal tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2810, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (ab2810).

  • All lanes : Anti-Rab9 antibody [Mab9] (ab2810) at 10 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-arginine, and sodium azide (ab2810).

References

ab237960 has not yet been referenced specifically in any publications.

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