Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.102 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rabbit
Product nameRabbit Angiopoietin-1 ELISA Kit
See all Angiopoietin 1 kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range0.102 ng/ml - 25 ng/ml
Sample specific recovery Sample type Average % Range Serum 106.6 75% - 140% Plasma 71.85 67% - 82% Cell culture media 85.4 67% - 109%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Rabbit
Rabbit Angiopoietin-1 ELISA Kit (ab273247) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of rabbit Angiopoietin-1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for rabbit Angiopoietin-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-rabbit Angiopoietin-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Tested applicationsSuitable for: Sandwich ELISAmore details
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Rabbit Angiopoietin-1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Rabbit Angiopoietin-1 Antibody 2 vials Rabbit Angiopoietin-1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
FunctionBinds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
Sequence similaritiesContains 1 fibrinogen C-terminal domain.
- Information by UniProt
- AGP 1
Our Abpromise guarantee covers the use of ab273247 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab273247 has not yet been referenced specifically in any publications.