Overview

  • Product name
    Rabbit Anti-Armenian hamster IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Rabbit
  • Target species
    Armenian hamster
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Armenian Hamster IgG and Armenian Hamster Serum.
  • Tested applications
    Suitable for: Dot blot, WB, ELISA, Electron Microscopy, IHC-P, ICCmore details
  • Immunogen

    Armenian Hamster IgG whole molecule.

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose.
  • Conjugation notes
    Horseradish Peroxidase (HRP).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
WB 1/1000 - 1/5000.
ELISA 1/25000. This product has been assayed against 1.0 ug of Armenian Hamster IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Electron Microscopy Use at an assay dependent concentration.
IHC-P 1/1000 - 1/5000.
ICC 1/1000 - 1/5000.

Images

  • ab5745 tested against purified Armenian Hamster IgG. Each well was coated in duplicate with 1.0 µg of Armenian Hamster IgG. The starting dilution of antibody was 5µg/ml and the X-axis represents the log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate

References

This product has been referenced in:
  • Boraschi-Diaz I  et al. Collagen type I degradation fragments act through the collagen receptor LAIR-1 to provide a negative feedback for osteoclast formation. Bone 117:23-30 (2018). Read more (PubMed: 30217615) »
  • Bouillez A  et al. MUC1-C integrates PD-L1 induction with repression of immune effectors in non-small-cell lung cancer. Oncogene 36:4037-4046 (2017). Read more (PubMed: 28288138) »
See all 7 Publications for this product

Customer reviews and Q&As

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Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Ab5745 staining Armenian hamster IgG by Immunohistochemistry (IHC-P) using the spleen of a healthy C57BL/6J mouse. Tissue was fixed with formalin. Antigen retrieval was by heat mediation in citrate buffer, carried out in a pressure cooker. Tissue sections were blocked with 2.5% goat serum for 2 hours at room temperature, followed by incubation with primary antibody (ab231545 1/100 in 1% BSA) overnight at 4°C. The secondary antibody (1/300 in 1% BSA) was added for 40 min at room temperature.
(The image shows the second antibody control stain for positive stain please see ab231545)

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Verified customer

Submitted Sep 04 2018

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