Key features and details
- Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Rabbit
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Product nameRabbit Anti-Chicken IgY H&L (Alkaline Phosphatase)
See all IgY secondary antibodies
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.00136% Zinc chloride, 1.19% HEPES, 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Conjugation notesMolar enzyme: antibody protein ratio is 1:1
General notesPart of the AbExcel range.
Our Abpromise guarantee covers the use of ab97137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/100 - 1/1000.|
|ELISA||1/5000 - 1/50000. (as Primary).|
|WB||1/5000 - 1/50000. (Chemiluminescent).|
All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 µg/ml
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Lanes 1-2 : Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase) (ab97137) at 1/5000 dilution
Lanes 3-4 : Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase) (ab97137) at 1/20000 dilution
Lanes 5-6 : Rabbit Anti-Chicken IgY H&L (Alkaline Phosphatase) (ab97137) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 12 minutes
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab97137 has not yet been referenced specifically in any publications.