Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
Key features and details
- Rabbit Anti-Chicken IgY H&L (Biotin)
- Conjugation: Biotin
- Host species: Rabbit
- Suitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, ICC/IF
Overview
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Product name
Rabbit Anti-Chicken IgY H&L (Biotin)
See all IgY secondary antibodies -
Host species
Rabbit -
Target species
Chicken -
Tested applications
Suitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, ICC/IFmore details -
Immunogen
chicken IgY whole molecule
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Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. -
Conjugation notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
Clonality
Polyclonal -
Research areas
Associated products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
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| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| WB |
1/2000 - 1/20000.
|
|
| Dot blot |
Use at an assay dependent concentration.
|
|
| ELISA |
1/500000.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/1000 - 1/5000.
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
WB
1/2000 - 1/20000. |
|
Dot blot
Use at an assay dependent concentration. |
|
ELISA
1/500000. |
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
1/1000 - 1/5000. |
Images
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Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
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Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (7)
ab6752 has been referenced in 7 publications.
- McKenzie JA et al. Loss of scleraxis in mice leads to geometric and structural changes in cortical bone, as well as asymmetry in fracture healing. FASEB J 31:882-892 (2017). PubMed: 27864378
- Weis B et al. Inhibition of intestinal tumor formation by deletion of the DNA methyltransferase 3a. Oncogene 0:N/A (2014). PubMed: 24837369
- Jiao TT et al. Importance of spondin 1 and cellular retinoic acid binding protein 1 in the clinical diagnosis of ovarian cancer. Int J Clin Exp Pathol 6:3036-41 (2013). IHC-P . PubMed: 24294399
- Gakis G et al. XPA-210: a new proliferation marker to characterize tumor biology and progression of renal cell carcinoma. World J Urol 29:801-6 (2011). IHC-P . PubMed: 21113600
- Wong EW et al. Regulation of blood-testis barrier dynamics by TGF-beta3 is a Cdc42-dependent protein trafficking event. Proc Natl Acad Sci U S A 107:11399-404 (2010). IHC-Fr . PubMed: 20534521
- Koryakina YA et al. Characterization of a panel of six beta2-adrenergic receptor antibodies by indirect immunofluorescence microscopy. Respir Res 9:32 (2008). ICC/IF . PubMed: 18423037
- Ergün S et al. Cell type-specific expression of LINE-1 open reading frames 1 and 2 in fetal and adult human tissues. J Biol Chem 279:27753-63 (2004). PubMed: 15056671
