Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)

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Overview

  • Product name
    Rabbit Anti-Chicken IgY H&L (Biotin)
    See all IgY secondary antibodies
  • Host species
    Rabbit
  • Target species
    Chicken
  • Tested applications
    Suitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, ICC/IFmore details
  • Immunogen

    chicken IgY whole molecule

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    Affinity purified
  • Purification notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/2000 - 1/20000.
Dot blot Use at an assay dependent concentration.
ELISA 1/500000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.

Images

  • Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    Ab6752 was used with the primary antibody ab13989 in ICC/IF. See Abreview on ab13989.
  • Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    Ab6752 was used at dilution 1/1000 with the primary antibody ab13970 in IHC-Fr. See the review on ab13970.

References

This product has been referenced in:
  • Weis B  et al. Inhibition of intestinal tumor formation by deletion of the DNA methyltransferase 3a. Oncogene 0:N/A (2014). Read more (PubMed: 24837369) »
  • Jiao TT  et al. Importance of spondin 1 and cellular retinoic acid binding protein 1 in the clinical diagnosis of ovarian cancer. Int J Clin Exp Pathol 6:3036-41 (2013). IHC-P . Read more (PubMed: 24294399) »
See all 6 Publications for this product

Publishing research using ab6752? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Do you know if it would be possible to add a %glycerol to this product and keep under -20 storage conditions unaliquoted? Thank you.

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Answer

Thank you for your enquiry. Antibodies can be stored with glyserol at -20oC. However, there is some evidence that this can sometimes decrease the activity of the antibody which means you may have to use the antibody at a higher concentration in your experiments. I hope this helps. Please get back to me if you have any more questions, and I will be happy to help. With thanks.

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Question

What about working dilutions of this antibody?

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Answer

We recommend the following dilutions: ELISA 1:5,000 - 1:20,000 Immunoblotting 1:3,000 - 1:10,000 Immunohistochemistry 1:100 - 1:1,000

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