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  1. Link

    rabbit-chicken-igy-hl-biotin-ab6752.pdf

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Secondary antibodies anti-Chicken IgY Biotin
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Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (9)

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Abpromise

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Find out more.

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
  • Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)

Key features and details

  • Rabbit Anti-Chicken IgY H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Rabbit
  • Suitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, ICC/IF

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Overview

  • Product name

    Rabbit Anti-Chicken IgY H&L (Biotin)
    See all IgY secondary antibodies
  • Host species

    Rabbit
  • Target species

    Chicken
  • Tested applications

    Suitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, ICC/IFmore details
  • Immunogen

    chicken IgY whole molecule

  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
  • Conjugation notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality

    Polyclonal
  • Research areas

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Biotin

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/2000 - 1/20000.
Dot blot
Use at an assay dependent concentration.
ELISA
1/500000.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.
Notes
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/2000 - 1/20000.
Dot blot
Use at an assay dependent concentration.
ELISA
1/500000.
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/1000 - 1/5000.

Images

  • Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    Ab6752 was used with the primary antibody ab13989 in ICC/IF. See Abreview on ab13989.
  • Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    Immunohistochemistry (Frozen sections) - Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
    Ab6752 was used at dilution 1/1000 with the primary antibody ab13970 in IHC-Fr. See the review on ab13970.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (9)

Publishing research using ab6752? Please let us know so that we can cite the reference in this datasheet.

ab6752 has been referenced in 9 publications.

  • López-Fernández A  et al. Effect of Allogeneic Cell-Based Tissue-Engineered Treatments in a Sheep Osteonecrosis Model. Tissue Eng Part A 26:993-1004 (2020). PubMed: 32122263
  • McKenzie JA  et al. Loss of scleraxis in mice leads to geometric and structural changes in cortical bone, as well as asymmetry in fracture healing. FASEB J 31:882-892 (2017). PubMed: 27864378
  • Ren J  et al. Imaging rhodopsin degeneration in vivo in a new model of ocular ischemia in living mice. FASEB J 30:612-23 (2016). PubMed: 26443823
  • Weis B  et al. Inhibition of intestinal tumor formation by deletion of the DNA methyltransferase 3a. Oncogene 0:N/A (2014). PubMed: 24837369
  • Jiao TT  et al. Importance of spondin 1 and cellular retinoic acid binding protein 1 in the clinical diagnosis of ovarian cancer. Int J Clin Exp Pathol 6:3036-41 (2013). IHC-P . PubMed: 24294399
  • Gakis G  et al. XPA-210: a new proliferation marker to characterize tumor biology and progression of renal cell carcinoma. World J Urol 29:801-6 (2011). IHC-P . PubMed: 21113600
  • Wong EW  et al. Regulation of blood-testis barrier dynamics by TGF-beta3 is a Cdc42-dependent protein trafficking event. Proc Natl Acad Sci U S A 107:11399-404 (2010). IHC-Fr . PubMed: 20534521
  • Koryakina YA  et al. Characterization of a panel of six beta2-adrenergic receptor antibodies by indirect immunofluorescence microscopy. Respir Res 9:32 (2008). ICC/IF . PubMed: 18423037
  • Ergün S  et al. Cell type-specific expression of LINE-1 open reading frames 1 and 2 in fetal and adult human tissues. J Biol Chem 279:27753-63 (2004). PubMed: 15056671

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Do you know if it would be possible to add a %glycerol to this product and keep under -20 storage conditions unaliquoted? Thank you.

Read More

Abcam community

Verified customer

Asked on Aug 04 2006

Answer

Thank you for your enquiry. Antibodies can be stored with glyserol at -20oC. However, there is some evidence that this can sometimes decrease the activity of the antibody which means you may have to use the antibody at a higher concentration in your experiments. I hope this helps. Please get back to me if you have any more questions, and I will be happy to help. With thanks.

Read More

Abcam Scientific Support

Answered on Aug 08 2006

Question

What about working dilutions of this antibody?

Read More

Abcam community

Verified customer

Asked on Jun 28 2001

Answer

We recommend the following dilutions: ELISA 1:5,000 - 1:20,000 Immunoblotting 1:3,000 - 1:10,000 Immunohistochemistry 1:100 - 1:1,000

Read More

Abcam Scientific Support

Answered on Jun 28 2001

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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