Rabbit Anti-Chicken IgY H&L (DyLight® 488) preadsorbed (ab96955)

Overview

  • Product name

    Rabbit Anti-Chicken IgY H&L (DyLight® 488) preadsorbed
    See all IgY secondary antibodies
  • Host species

    Rabbit
  • Target species

    Chicken
  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig and rat was detected.

     

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity


    Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • Conjugation

    DyLight® 488. Ex: 493nm, Em: 518nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antiserum was cross adsorbed using bovine, goat, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References

ab96955 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply. A549 cells were recommended because they showed weak to no staining using a similar antibody.  Plus these are the results found in the Human Protein Atlas: http://www.proteinatlas.org/ENSG00000169252/cell/A-549 I'm not sure if I have a clear explanation as to why these stained well because fewer than 5% were shown to have a signal in the Human Protein Atlas, unless the antibody was binding to something non-specifically.  Did you run a no-primary control?   Let me know how the staining goes with the mouse keratinocytes.  I look forward to your reply.

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Answer

Thank you for your reply and for providing the requested information. After seeing the data and your protocol, I would like to make some suggestions that may improve your results. One of my colleagues who has worked with chicken antibodies in the past has found them to be a bit "sticky" and suggested the following: Increase the amount of serum and blocking time. I see that for non-permeabilized cells you use 10% serum - I would advice increasing to 10% and incubating for 1-2 hours. Titrating the antibody concentrations - You appear to get a nice specific signal at 1:250. Continue to titrate that so that you still observe the specific signal and reduce the non-specific signal from the isotype control. Overall, the background is not too bad and this should be easy to titrate. I have contacted the laboratory regarding a negative control and will forward that information to you as soon as possible. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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