Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)

Submit a review Q&A (2)References (18)

Overview

  • Product name
    Rabbit Anti-Chicken IgY H&L (HRP)
    See all IgY secondary antibodies
  • Host species
    Rabbit
  • Target species
    Chicken
  • Tested applications
    Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • Immunogen

    chicken IgY whole molecule

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    Do not add Sodium Azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Horseradish Peroxidase (HRP).
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes

    May cross-react with chicken IgM

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent concentration.
ELISA 1/145000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/2500 - 1/10000.

Images

  • Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
    Immunohistochemistry (Frozen sections) - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
    Ab6753 was used at dilution 1/2000 with the primary antibody ab16293 in IHC-Fr. See the review on ab16293.
  • ELISA - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
    ELISA - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
    Ab6753 was used at dilution 1/10000 with the primary antibody ab15879 in ELISA. See the review on ab15879.
  • Western blot - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
    Western blot - Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab6753)
    Ab6753 was used at dilution 1/5000 with the primary antibody ab19130 in WB. See the review on ab19130.

References

This product has been referenced in:
See all 18 Publications for this product

Publishing research using ab6753? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Phoned because the customer is seeing very high background on the blot everywhere but the lanes which contain sample. Still getting good, clean signal on sample but was curious about this response.

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Answer

Thank you for contacting us.

I have had the opportunity to speak with my collegues regarding this issue.We have seen similar patterns when the gel is over-heating during transfer or contaminants are present in the transfer buffer or equipment. So we definitely recommend using all new buffers and doing your transfer a lower/colder conditions.

I also wanted to suggest using a no primary antibody control to see if you experience the same results.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Recently, I bought 5 antibodies from abcam, which are: 1 x GABARAPL2 antibody(ab15858) 1 x CREB antibody (ab5803) 1 x Leptin Receptor antibody (ab7211) 1 x GABARAP antibody (ab1133) 1 x Chicken IgY (H&L) antibody (ab6753) The order reference number is 66504 (PON: L-15) I receive them yesterday and 3 of them were OK, but I think that there was some kind of trouble with two of them, GABARAPL2 antibody (ab15858) and GABARAP antibody (ab1133). I was expected to receive 50 uL of GABARAPL2 antibody and I only received 40 uL. In the case of GABARAP antibody I was expected to recieve 100 uL and I have only received 40 uL. In this case, the recomended dilution for this antibody is 1:500, so I probably won't be able to establish the optimal assay conditions for that antibody with the supplied amount of it. I write you to ask for a solution for this event, because I have paid for a determined amount of antibody and I have received less than the half.

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Answer

Le pido que nos disculpe por la falta en su orden. He hecho una nueva orden cual le sera enviada lo mas pronto posible. Porfavor refiera al numero de orden 68450 si necesita mas información sobre el estado de su orden. Otra vez, porfavor disculpe la falta que recibio. I offer you my sincerest apologies for the shortage you've received. I have gone ahead and set up a replacement order to be shipped to you as soon as possible. Please reference order number 68450 if you require further information about the status of your order. Once again, I apologize for the shortage you received.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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