Overview

  • Product name

    Rabbit Anti-Cow IgG H&L (beta-galactosidase)
    See all IgG secondary antibodies
  • Description

    Rabbit polyclonal Secondary Antibody to Cow IgG - H&L (BGAL)
  • Host species

    Rabbit
  • Target species

    Cow
  • Specificity

    Beta-galactosidase labelling is well adapted to ELISA and produces no or very little background. It is more difficult to use in immunochemistry where its use can be justified only when the endogenous activities of other available labels produce significant problems.
  • Tested applications

    Suitable for: ELISA, IHC-Fr, ICC, WBmore details
  • Immunogen

    Highly purified whole bovine immunoglobulin

  • Conjugation

    beta-galactosidase

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab136710 is purified by a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Preparation of Substrate Solutions: 0.1M phosphate buffer pH7, 1mM MgSO4, 0.2mM MnSO4, 2mM Mg titriplex. Add extemporaneously 7µl/ml beta-mercaptoethanol and 0.9mg/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V/2 volume of 1M Na2CO3.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100 - 1/1000.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

References

ab136710 has not yet been referenced specifically in any publications.

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