Product nameRabbit F(ab) Anti-Horse IgG H&L (FITC)
See all IgG secondary antibodies
Tested applicationsSuitable for: ICC, ICC/IF, IHC-Fr, IHC-P, Flow Cytmore details
Highly purified whole immunoglobulin from horse
ConjugationFITC. Ex: 493nm, Em: 528nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesFab and F(ab')2 are respectively mono and bivalent fragments of immunoglobulins, i.e. including one or two sites for the fixation of the antigen. They are prepared by enzymic digestion (papain or pepsin). They are purified by affinity chromatography and are thus particularly specific.
Fab and F(ab')2 fragments have certain advantages with respect to the entire antibodies in immunohistochemistry, immunocytology and flow cytometry tests.
On the one hand their specificity is increased since they can no longer be bound in a non-specific way by their Fc part as entire Ig’s are.
On the other hand their use also increase the sensitivity of the technique. In fact, their small size enhances their penetration into tissues and cells as well as their availability for the antigens (this is so particularly for Fab).
Finally, conjugate Fab's have a label/antigenic ratio that is twice as much compared to antibodies (except fluorescent conjugates).
Conjugation notesThe F/P (fluorochrome/protein) molar ratio is set between 3 and 7.
Our Abpromise guarantee covers the use of ab136729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/10 - 1/100.|
|ICC/IF||1/50 - 1/200.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
ab136729 has not yet been referenced specifically in any publications.